张宇,孔凡栋,马青云,等.海洋真菌Aspergillus sp. SCS-KFD03化学成分研究[J].中国海洋药物,2017,36(2):15-21.
海洋真菌Aspergillus sp. SCS-KFD03化学成分研究
Study on the chemical constituents from the marine-derived fungus Aspergillus sp. SCS-KFD03
投稿时间：2016-08-19  修订日期：2016-10-14
DOI：
中文关键词:  海洋真菌  曲霉属  化学成分  乙酰胆碱酯酶抑制活性  α-糖苷酶抑制活性
English Keywords:Marine-derived fungus  Aspergillus sp.  Chemical composition  Acetylcholinesterase inhibitory activity  α-Glucosidase inhibitory activity
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作者单位E-mail
张宇 海南大学海洋学院/热带生物资源教育部重点实验室 stzhangyu92@163.com 
孔凡栋 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室  
马青云 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室  
黄圣卓 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室  
周丽曼 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室  
戴好富 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室  
邓世明 海南大学海洋学院/热带生物资源教育部重点实验室  
赵友兴* 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室 zhaoyouxing@itbb.org.cn 
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中文摘要:
      目的 对海洋真菌Aspergillus sp. SCS-KFD03的发酵液中化学成分进行分离鉴定，并测定其生物活性。 方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等技术进行分离纯化，运用各种波谱方法对分离所得化合物进行结构鉴定，并测定化合物乙酰胆碱酯酶和α-糖苷酶抑制活性。结果 分离鉴定了9个化合物，经鉴定为phomaligol A1（1）、5-（乙酰氧基甲基）呋喃-3-酸（2）、 phomapyrone C（3）、4-羟基异苯并呋喃-1(3H)-酮（4）、penicillivinacine（5）、22(E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol（6）、lucidal（7）、dankasterone A（8）、bis-(2-methylheptyl)-phthalate（9），其中化合物4为新天然产物。化合物5、6、7和9具有较弱的乙酰胆碱酯酶抑制的活性，化合物5具有强于阳性药阿卡波糖的α-糖苷酶抑制活性，8和9的α-糖苷酶抑制活性与阳性药相当。结论 化合物1~9均为首次从海洋曲霉属真菌Aspergillus sp. SCS-KFD03中分离得到，部分化合物具有乙酰胆碱酯酶和α-糖苷酶的抑制活性。
English Summary:
      Objective To study the chemical constituents from the fermentation broth of marine-derived fungus Aspergillus sp. strain SCS-KFD03. Methods The constituents were isolated and purified by Silica gel, Sephadex LH-20 column chromatography and HPLC chromatography and identified by spectral data analyses, while their acetylcholinesterase and α-glucosidase inhibitory activities were evaluated. Results Nine compounds were isolated from the marine-derived fungus Aspergillus sp.SCS-KFD03 and identified as phomaligol A1 (1), 5- (acetoxymethyl) furan-3 acid (2), phomapyrone C (3), 4-hydroxyisobenzofuran-1(3H)-one (4), penicillivinacine (5), 22(E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (6), lucidal (7), dankasterone A (8), bis-(2-methylheptyl)-phthalate (9). Among the compounds, compound 4 is a new natural product. The acetylcholinesterase and α-glucosidase inhibitory activities of these compounds were evaluated at the first time. Compounds 5, 6, 7, and 9 showed weak inhibitory activities against acetylcholinesterase. Compound 5 has stronger inhibitory activity against α-glucosidase than the positive drug acarbose, while compounds 8 and 9 exhibited inhibitory activity against α-glucosidase as strong as the positive drug. Conclusion All the compounds were obtained from the Aspergillus sp. SCS-KFD03 for the first time, and some of them had a certain inhibitory activity on acetylcholinesterase and α-glucosidase.
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