郭黎,周路雨,蔡黎娟,等.海绵共附生真菌Purpureocillium lilacinum的化学成分及T细胞调节活性研究[J].中国海洋药物,2024,43(2):53-59.
海绵共附生真菌Purpureocillium lilacinum的化学成分及T细胞调节活性研究
Structure and T cell regulatory activity of metabolites from the sponge-associated fungi Purpureocillium lilacinum
投稿时间:2024-02-29  修订日期:2024-03-26
DOI:10.13400/j.cnki.cjmd.2024.02.011
中文关键词:  海绵共附生真菌  甾体  T细胞调节活性
English Keywords:sponge associated fungi  steroid  T cells regulate activity
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作者单位E-mail
郭黎 同济大学 医学院 2356488928@qq.com 
周路雨 同济大学 医学院 zly976@outlook.com 
蔡黎娟 同济大学 医学院 ljuancai@163.com 
韩华 同济大学 医学院 hanhua@tongji.edu.cn 
张文* 同济大学 医学院 wenzhang1968@163.com 
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中文摘要:
      目的 对海绵Hymeniacidon sp.共附生真菌Purpureocillium lilacinum的化学成分及T细胞调节活性进行研究。方法 运用硅胶柱色谱、凝胶柱色谱及高压液相色谱等色谱技术对真菌乙酸乙酯粗提物进行分离纯化得到单体化合物,通过现代波谱(核磁共振,质谱)及光谱(旋光)的综合运用并结合文献对照对化合物结构进行鉴定,运用流式实验对化合物T细胞调节活性进行测试。结果 共分离获得5个甾体化合物,鉴定为(24R)-豆甾-4-烯-6β-醇-3-酮(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,9α-三醇-6-酮(2)、(22E,24R)-麦角甾-7,22-二烯-3β,5α-二醇-6-酮(3)、麦角甾-5,24-二烯-3β-醇-7,23-二酮(4)及(22E,24R)-麦角甾-5,8,22-三烯-3β-醇-7-酮(5)。体外活性筛选实验中,化合物2~5在2.5 μmol/L时对刀豆蛋白A(ConA)诱导的T细胞增殖显示抑制活性,化合物5在10 μmol/L时对CD3+T细胞的增殖显示显著抑制活性,所有受试化合物对CD4+/CD8+ T细胞分化活性均无影响。结论 化合物1~4为首次从P. lilacinum中分离获得。本文是对化合物1~5 T细胞调节活性的首次报道。
English Summary:
      Objective To investigate the structure and T cell regulatory activity of metabolites from the fungi Purpureocillium lilacinum associated with the sponge Hymeniacidon sp. Methods The ethyl acetate crude extract of the fungi was purified by silica gel and Sephadex LH-20 column chromatography and high pressure liquid chromatography. The structures of the compounds were identified by the modern spectroscopic methods (nuclear magnetic resonance, mass spectrometry and optical rotation) in combination with the data reported in literature. The T cell regulatory activity of the compounds was tested by flow assay. Results Five steroids were isolated from the fungi P. lilacinum and identified as (24R)-stigmast-4-en-6β-ol-3-one (1), (22E,24R)-ergosta-7,22-dien-3β,5α,9α-triol-6-one (2), (22E,24R)-ergosta-7,22-dien-3β,5α-diol-6-one (3), ergosta-5,24-dien-3β-ol-7,23-diketone (4) and (22E,24R)-ergosta-5,8,22-trien-3β-ol-7-one (5). In an in vitro bioscreening, compound 2~5 showed inhibitory activity at 2.5 μmol/L on T cell proliferation induced by concanavalin A (ConA). In particular, compound 5 showed significant inhibitory activity on CD3+T cell proliferation at 10 μmol/L. The tested compounds showed no activity toward the CD4+/CD8+ ratio. Conclusion Compounds 1~4 were reported for the first time from the fungi P. lilacinum. This is the first report on the T cell regulatory activity of these compounds.
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