胡晓燕,苟春霞,卢立明,等.黄河三角洲来源真菌Fusarium oxysporum RD-79次级代谢产物研究[J].中国海洋药物,2024,43(2):79-84.
黄河三角洲来源真菌Fusarium oxysporum RD-79次级代谢产物研究
Studies on Secondary Metabolites and Activities of the Fungus Fusarium oxysporumRD-79 from the Yellow River Delta
投稿时间:2023-11-02  修订日期:2024-01-09
DOI:10.13400/j.cnki.cjmd.2024.02.009
中文关键词:  黄河三角洲  真菌  次级代谢产物  一氧化氮抑制活性
English Keywords:Yellow River Delta  Fungus  secondary metabolites  NO inhibitory activity
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作者单位E-mail
胡晓燕 滨州医学院附属医院药学部 byfyhxy@126.com 
苟春霞 滨州医学院附属医院药学部 373773381@qq.com 
卢立明 滨州医学院附属医院药学部 294293650@qq.com 
苗双 滨州医学院附属医院医学研究中心 1038898609@qq.com 
宫凯凯* 滨州医学院附属医院医学研究中心 gongkaikai1005@163.com 
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中文摘要:
      研究一株黄河三角洲滨海湿地来源的真菌Fusarium oxysporum RD-79的次级代谢产物及其生物活性。利用硅胶柱色谱、ODS柱色谱、半制备高效液相色谱等分离方法对该菌株发酵产物进行分离纯化,通过核磁共振(NMR)、质谱(MS)等方法,结合相关文献对比鉴定化合物结构;分别采用CCK8法和Griess法对化合物进行抗肿瘤活性和抗炎活性测试。从Fusarium oxysporum RD-79的发酵产物中分离得到8个单体化合物,分别为5-bμtyl-4-methoxy-6-methyl-2H-pyran-2-one(1)、甲醛甲苯(2)、delitpyroneD(3)、3-hydroxymethyl-6,8-dimethoxycoμmaarin(4)、5-[(1S,2S)-1,2-dihydroxybμtyl]-4-methoxy-6-methyl-2H-pyran-2-one(5)、setosphapyroneA(6)、5-(1-hydroxybμtyl)-4-methoxy-6-methyl-2H-pyran-2-one(7)、9-O-methylscytalol A(8)。化合物1~8均可不同程度抑制NO的产生;在浓度为100μg/mL下,化合物1~8均无抗肿瘤活性。
English Summary:
      To study the secondary metabolites and their biological activities of a fungus FusariumoxysporumRD-79 from the coastal wetland of the Yellow River Delta. The fermentation products of the strain were separated and purified by silica gel column chromatography, ODS column chromatography, semi-preparative high performance liquid chromatography and other separation methods.Their structures were elucidated based on the spectral data ofnuclear magnetic resonance analyses (NMR) and mass spectrometry (MS) and compared with literature data. CCK8 and Griess method were used to test the cytotoxic activity and anti-inflammatory activity of the compounds. Eight pure compounds were isolated and identified as 5-bμtyl-4-methoxy-6-methyl-2H-pyran-2-one (1), 2-methylbenzaldehyde (2), delitpyrone D (3), 3-hydroxymethyl-6-8-dimethoxycoμmaarin (4), 5-[(1S,2S)-1,2-dihydroxybμtyl]-4-methoxy-6-methyl-2H-pyran-2-one (5), setosphapyrone A (6), 5-(1-hydroxybμtyl)-4-methoxy-6-methyl-2H-pyran-2-one (7), 9-O-methylscytalol A (8) respectively. Compounds 1~8 could inhibit the production of NO in varying degrees. At the concentration of 100 μg/mL, compounds 1~8 showed no anti-tumor activity.
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