周康,刘冬云,王斌,等.南海软珊瑚Lobophytum sp.中二萜类化合物的研究[J].中国海洋药物,2024,43(2):60-66.
南海软珊瑚Lobophytum sp.中二萜类化合物的研究
Studies on diterpenoids of soft coral Lobophytum sp. from South China Sea
投稿时间:2023-01-07  修订日期:2023-03-14
DOI:10.13400/j.cnki.cjmd.2024.02.007
中文关键词:  软珊瑚  次级代谢产物  西松烷型二萜  抗炎活性  Lobophytum sp.
English Keywords:soft coral  Secondary metabolites  Cembranoids  anti-inflammatory activity  Lobophytum sp.
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作者单位E-mail
周康 浙江海洋大学 m18806851480@163.com 
刘冬云 北京大学 天然药物及仿生药物国家重点实验室 ldy83@bjmu.edu.cn 
王斌 浙江海洋大学 食品与药学学院 wangbin@zjou.edu.cn 
程伟* 北京大学 天然药物及仿生药物国家重点实验室 chengwei@bjmu.edu.cn 
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中文摘要:
      目的 对海洋来源软珊瑚Lobophytum sp.中的化学成分结构多样性及生物活性进行研究。方法 利用硅胶、十八烷基硅烷键合硅胶(ODS-C18)、凝胶Sephadex LH-20和半制备高效液相(semi-HPLC)等色谱学方法对软珊瑚乙酸乙酯提取物中的化学成分进行分离;利用紫外(UV)、核磁共振波谱(NMR)、质谱(MS)等波谱学方法,结合与文献的数据对比确定化合物的结构。结果 从软珊瑚的乙酸乙酯提取物中分离鉴定了10个西松烷型二萜类化合物,分别为sarcomililatins B(1)、lobophytins A(2)、isosarcophine(3)、sarcophytoxide(4)、isosarcophytoxide(5)、(+)-11,12-epoxyepoxy-11,12-dihydrocembrene-C(6)、cherbonnolide B(7)、sarcomililatins A(8)、sarcophytonin B(9)和3,4-dihydro-4α-hydroxy-?2-sarcophine(10)。其中化合物7和8为首次从该属软珊瑚中分离得到。化合物1和3在20 μmol/L 浓度下对LPS诱导NO产生的抑制率分别为31.5%和28.6%。结论 从南海软珊瑚Lobophytum sp.中分离得到了化合物1~10,其中化合物1和3对LPS诱导NO产生具有一定的抑制作用。
English Summary:
      Objective To investigate the structural diversity and biological activity of chemical constituents from soft coral lobophytum sp.. Methods The compounds were isolated from crude extract and purified by silica gel column, ODS-C18 column, Sephadex LH-20 and HPLC. Their structures were determined by analysis of UV, NMR, MS data and comparison with those reported in literatures. Results Ten cembrane diterpenoids were isolated and identified as sarcomililatins B (1), lobophytins A (2), isosarcophine (3), sarcophytoxide (4), isosarcophytoxide (5), (+)-11,12-epoxyepoxy-11,12-dihydrocembrene-C (6), cherbonnolide B (7), sarcomililatins A (8), sarcophytonin B (9) and 3,4-dihydro-4α-hydroxy-?2-sarcophine (10). Compounds 7 and 8 were isolated from this genus for the first time. At a concentration of 20 μmol/L, compounds 1 and 3 exhibited inhibition of LPS-induced NO production with inhibitory rates as 31.5% and 28.6%, respectively. Conclusion Ten cembrane diterpenoids were isolated from soft corals Lobophytum sp.. Compounds 1 and 3 showed inhibition effects on NO production induced by LPS.
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