蔡瑾,王林祥,张子怡,等.一株药用红树内生真菌Talaromyces flavus TGGP34次级代谢产物及其活性研究[J].中国海洋药物,2021,40(6):37-43.
一株药用红树内生真菌Talaromyces flavus TGGP34次级代谢产物及其活性研究
Study on the secondary metabolites and bioactivities of a medicinal mangrove endophytic fungus Talaromyces flavus TGGP34
投稿时间:2021-02-22  修订日期:2021-05-01
DOI:
中文关键词:  内生真菌  Talaromyces flavus  次级代谢产物  抗菌活性
English Keywords:endophytic fungus  Talaromyces flavus  secondary metabolites  antibacterial activity.
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作者单位E-mail
蔡瑾 海南师范大学 1912246490@qq.com 
王林祥 海南师范大学化学与化工学院 2524660107@qq.com 
张子怡 海南师范大学化学与化工学院 2524660107@qq.com 
李姗姗 海南师范大学化学与化工学院 2628744649@qq.com 
陈敏静 海南师范大学 热带药用资源化学教育部重点实验室 3546795109@qq.com 
罗运展 海南师范大学 热带药用资源化学教育部重点实验室 3349158951@qq.com 
郑彩娟 海南师范大学 热带药用资源化学教育部重点实验室 caijuan2002@163.com 
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中文摘要:
      目的 研究1株药用红树老鼠簕(Acanthus ilicifolius L.)来源内生真菌篮状菌属的Talaromyces flavus TGGP34的次级代谢产物及其抗菌活性。方法 利用正、反相硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备高效液相色谱(HPLC)等色谱分离方法,对TGGP34发酵产物的乙酸乙酯粗浸膏进行系统分离纯化;利用核磁共振波谱(NMR)、质谱(MS)等方法并结合文献报道数据确定化合物结构,并对其抗菌活性进行测定。结果 从内生真菌T. flavus TGGP34中分离得到9个化合物,包括4个甾醇类化合物β-sitosterol(1)、24-methylcholesta-7,22-diene-3β,5α,6β-triol(2)、3β,15β-dihydroxyl-(22E,24R)-ergosta-5,8(14),22-trien-7-one(3)、ganodermaside B(4),3个内酯类化合物dehydroisopenicillide(5)、penicillide(6)、purpactin A(7),2个二酮哌嗪类化合物cyclo-(S-Pro-R-Val)(8)和cyclo-(L-Pro-L-Leu)(9),其中化合物3对3株致病菌金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)和白色念珠菌(Candida albicans)表现出明显的抑制活性,其MIC值分别为12.5、12.5和6.25 μg/mL。结论 化合物1~4为首次从篮状菌属(Talaromyces sp.)中分离得到;化合物3具有显著的抗菌活性,具有重要的研究和潜在开发价值。
English Summary:
      Objective To study the secondary metabolites and biological activities of the endophytic fungus Talaromyces flavus TGGP34 derived from Acanthus ilicifolius L. Methods The isolated compounds were separated by normal and reverse phase silica gel, Sephadex LH-20, semi-preparative HPLC column chromatography, thin layer chromatography and so on. And their structures were identified by 1D NMR, MS, other spectral analysis methods and comparison with literature data. They were also tested for their antibacterial activity. Results Nine known compounds were isolated from the endophytic fungus T. flavus TGGP34, including four sterols β-sitosterol (1), 24-methylcholesta-7,22-diene-3β,5α,6β-triol (2), 3β,15β-dihydroxyl- (22E,24R)- ergosta-5,8(14), 22-trien-7-one (3), ganodermaside B (4), three lactones dehydroisopenicillide (5), penicillide (6), purpactin A (7), and two diketopiperazines cyclo-(S-Pro-R-Val) (8) and cyclo(L-Pro-L-Leu) (9). Compound 3 showed antibacterial activity against three pathogenic bacteria Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans, with the MIC values of 12.5, 12.5 and 6.25μg/mL, respectively. Conclusion Compounds 1-4 were isolated from the genus Talaromyces sp. for the first time, and compound 3 showed significant antibacterial activity, and had important research and potential development value.
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