| 黄琳洪,宋雪,刘国平,等.芋螺毒素Qc-VB-01的合成与抗菌活性研究[J].中国海洋药物,2025,(2):-. |
| 芋螺毒素Qc-VB-01的合成与抗菌活性研究 |
| Synthesis and antibacterial effect of conotoxin Qc-VB-01 |
| 投稿时间：2023-12-08 修订日期：2023-12-29 |
| DOI： |
| 中文关键词: 芋螺毒素、抗菌肽、固相肽合成、微量肉汤稀释法 |
| English Keywords:conotoxin, antimicrobial peptide, solid phase peptide synthesis, microbroth dilution method |
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| 中文摘要: |
| 目的:对从蠕食性橡果螺(Conus quercinus)获得的全新的芋螺毒素序列Qc-VB-01进行合成和抗菌活性评价。方法:利用固相肽合成方法合成线性肽，2,2-二硫二吡啶氧化法连接二硫键，以微量肉汤稀释法测试该多肽对5种致病好氧菌的最低抑菌浓度（MIC）。结果:成功合成芋螺毒素Qc-VB-01及其突变体M10A、M16A，利用质谱验证其分子量和分析液相证明其纯度大于95%。发现Qc-VB-01具有一定抗菌活性，尤其对于金黄色葡萄球菌CMCC(B) 26003效果显著。甲硫氨酸替换为丙氨酸后，不影响活性结果。结论:Qc-VB-01是具有一定抗菌活性的芋螺毒素，结构新奇简单而抗菌效果明显。 |
| English Summary: |
| Objective: To synthesize a novel conotoxin Qc-VB-01 obtained from Conus quercinus and evaluate its antibacterial activity. Methods: The linear peptide was synthesized by solid phase peptide synthesis method, the disulfide bond was linked by 2, 2-disulfide dipyridine oxidation method, and the minimum inhibitory concentration (MIC) of the peptide against 5 pathogenic aerobic bacteria was tested by microbroth dilution method. Results: Conotoxin Qc-VB-01 and its mutants M5A and M11A were synthesized successfully. Their molecular weight was verified by mass spectrometry and their purity was more than 95% by liquid phase analysis. It was found that Qc-VB-01 had good antibacterial activity, especially against Staphylococcus aureus CMCC(B) 26003. The replacement of methionine with alanine did not affect the activities of these mutants. Conclusion: Qc-VB-01 is a antibacterial peptide with a novel and simple structure. |
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