| 苏梓昕,刘梦珍,郑伊诺,等.海绵Hymeniacidon sp.共附生真菌Aspergillus sp.LFHM22的化学成分及抗炎活性研究[J].中国海洋药物,2027,(2):-. |
| 海绵Hymeniacidon sp.共附生真菌Aspergillus sp.LFHM22的化学成分及抗炎活性研究 |
| Study on the chemical constituents and anti-inflammatoryactivities of the fungus Aspergillus sp. LFHM22 associated with the sponge Hymeniacidon sp. |
| 投稿时间：2026-02-06 修订日期：2026-03-14 |
| DOI： |
| 中文关键词: 海绵共附生真菌，抗炎活性 |
| English Keywords:sponge-associated fungi anti-inflammatory activity. |
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| 中文摘要: |
| 目的 对海绵Hymeniacidon sp.共附生真菌Aspergillus sp. LFHM22的化学成分及生物活性进行研究。方法 采用硅胶柱层析、薄层色谱、Sephadex LH-20凝胶柱层析及高效液相色谱（HPLC）等多种色谱分离技术，对真菌发酵提取物（乙酸乙酯粗提物）进行系统分离与纯化，获得单体化合物；综合运用核磁共振（NMR）、质谱（MS）及旋光光谱等现代波谱方法，并结合文献数据比对进行化合物结构鉴定。采用CCK8法评价细胞存活率，采用Griess法评价化合物的抗炎活性。结果 从真菌Aspergillus sp. LFHM22中共分离得到12个化合物，分别为属于肽类的asperphenamate（1）、7-OMe-asperphenamate（2）、(S)-(-)-N-benzoylphenylalaninol（3）；属于生物碱类的penilumamide A（4）、penilumamide C（5）、penilumamide B（6）；属于aspochalasin类的aspochalasin H（7）、aspochalasin I（8）、aspochalasin D（9）、aspergillin PZ（10）、aspochalasin E（11）、aspergilluchalasin（12）。其中化合物9、10、12表现出显著细胞毒活性，1～2，5～8对LPS诱导的RAW 264.7细胞生成的NO具有显著的抑制活性。结论首次发现化合物5、6有显著的抗炎活性，且化合物8的抗炎活性优于阳性对照药地塞米松。 |
| English Summary: |
| Objective This study aimed to investigate the chemical constituents and biological activities of the epiphytic and endophytic fungus Aspergillus sp. LFHM22 associated with the sponge Hymeniacidon sp. Methods The ethyl acetate crude extract of the fermented broth from Aspergillus sp. LFHM22 was systematically separated and purified to obtain monomeric compounds by various chromatographic techniques, including silica gel column chromatography, thin-layer chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatography (HPLC). The structures of the isolated compounds were identified by comprehensive application of modern spectroscopic methods such as nuclear magnetic resonance (NMR), mass spectrometry (MS) and optical rotation spectroscopy, combined with comparison with literature data. Subsequently, the cell viability and anti-inflammatory activity of the compounds were evaluated by the CCK-8 assay and Griess assay, respectively. Results A total of 12 compounds were isolated from Aspergillus sp. LFHM22. Compounds 1–3 were peptide derivatives, identified as asperphenamate (1), 7-OMe-asperphenamate (2) and (S)-(-)-N-benzoylphenylalaninol (3). Compounds 4–6 were alkaloids, namely penilumamide A (4), penilumamide C (5) and penilumamide B (6). Compounds 7–12 belonged to aspochalasin-type derivatives, identified as aspochalasin H (7), aspochalasin I (8), aspochalasin D (9), aspergillin PZ (10), aspochalasin E (11) and aspergilluchalasin (12). Among them, compounds 9, 10 and 12 exhibited significant cytotoxic activity, while compounds 1–2 and 5–8 showed potent inhibitory activity against nitric oxide (NO) production in LPS-induced RAW 264.7 cells. Conclusion Compound 5 and 6 were found to exhibit significant anti-inflammatory activity for the first time, and the anti-inflammatory activity of Compound 8 was superior to that of dexamethasone, the positive control. |
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