| 高姗,孙俐丽,李亚,等.金枪鱼血合肽产品对大鼠缺铁性贫血的治疗效果研究[J].中国海洋药物,2027,(1):-. |
| 金枪鱼血合肽产品对大鼠缺铁性贫血的治疗效果研究 |
| Therapeutic Effects of Tuna Dark Muscle-Derived Peptide Products on Iron-Deficiency Anemia in Rats |
| 投稿时间：2025-05-26 修订日期：2026-01-09 |
| DOI： |
| 中文关键词: 金枪鱼血合肽 缺铁性贫血 抗氧化 新型肽基补铁制剂 |
| English Keywords:Tuna Dark Muscle-Derived Peptide Iron deficiency anemia Antioxidant Novel peptide-based iron supplement. |
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| 中文摘要: |
| 目的 评估金枪鱼血合肽系列产品(精制肽、粗制肽及复合肽)对缺铁性贫血(IDA)的治疗效果,并揭示其作用机制。方法 采用氨基酸组分分析探究其潜在的活性成分,构建IDA大鼠模型,通过血液学参数(血红蛋白、红细胞计数、红细胞压积、平均红细胞体积、血小板计数)、生长发育指标(体质量变化、脏器指数)以及氧化应激相关指标(组织铁含量、总抗氧化能力、超氧化物歧化酶活性、铁调素水平及血清总铁结合力)对产品的IDA治疗效果进行系统性评价及作用机制分析。结果 氨基酸分析结果显示,相较于对照组,各肽产品组氨基酸组成差异显著(p < 0.05或p < 0.01),其中精制肽组谷氨酸(Glu,14.51 g/100 g,较对照组提高387%)、甘氨酸(Gly,5.04 g/100 g,提高85%)和半胱氨酸(Cys,0.47 g/100 g)等关键功能氨基酸的显著富集,通过形成可溶性铁复合物、提供血红素合成前体及增强抗氧化能力等多重机制,有效提升了铁的生物利用度,为开发新型抗贫血肽制剂提供了理论依据；IDA大鼠模型实验结果表明,金枪鱼血合肽产品(尤其是精制肽和复合肽)在改善血液指标、脏器功能和铁代谢方面表现出显著优势；与模型对照组相比,精制肽组和复合肽组显著提高了血红蛋白水平(p < 0.01)、红细胞计数和红细胞压积,同时改善了肝脏、肾脏和脾脏功能指标(p < 0.05)；在抗氧化能力方面,精制肽组的总抗氧化能力(4.34 mM)和超氧化物歧化酶活力(158.22 U/mgprot)显著优于其他治疗组(p < 0.05)；铁代谢结果显示,复合肽组的血清总铁结合力值(127.4 μmol/L)与正常组无显著差异(p > 0.05),表明其具有更好的铁稳态调节作用。实验组(包括精制肽组、粗制肽组及复合肽组)铁调素水平与正常组无显著差异(p > 0.05),表明实验组具有恢复铁调素至正常生理水平的能力,尤以精制肽组效果最为显著。结论 金枪鱼血合肽产品在改善IDA方面展现出优于传统硫酸亚铁补充剂和阳性对照的治疗效果,尤以精制肽效果最佳,该研究结果为开发新型肽基补铁制剂提供了理论依据。 |
| English Summary: |
| Objective To evaluate the therapeutic effects and mechanisms of Tuna Dark Muscle-Derived Peptide Products (decolorized peptide, non-decolorized peptide, and composite peptide) on iron deficiency anemia (IDA). Methods Amino acid composition analysis was conducted to explore potential active ingredients. IDA rat models were established, and the therapeutic effects of the products on IDA were systematically evaluated through hematological parameters (hemoglobin, red blood cell count, hematocrit, mean corpuscular volume, platelet count), growth and development indicators (body weight changes, organ index), and oxidative stress-related indicators (tissue iron content, total antioxidant capacity, superoxide dismutase activity, hepcidin level, and serum total iron binding capacity). Results The findings demonstrate that compared to the control group, the peptide product groups exhibited significant differences in amino acid composition (p < 0.05 or p < 0.01). Notably, the refined peptide group showed remarkable enrichment of key functional amino acids including glutamic acid (Glu, 14.51 g/100 g, 387% increase versus control), glycine (Gly, 5.04 g/100 g, +85%), and cysteine (Cys, 0.47 g/100 g). These compositional advantages synergistically enhance iron bioavailability through multiple mechanisms: forming soluble iron complexes, providing heme synthesis precursors, and strengthening antioxidant capacity, thereby providing a theoretical foundation for developing novel anti-anemia peptide preparations.The IDA rat model experimental results demonstrated that Tuna Dark Muscle-Derived Peptide Products (especially the decolored peptide and composite peptide) showed significant advantages in improving blood indicators, organ function, and iron metabolism. Compared to the model control group, the decolored peptide group and composite peptide group significantly increased hemoglobin levels (p < 0.01), red blood cell count, and hematocrit, while also improving liver, kidney, and spleen function indicators (p < 0.05). In terms of antioxidant capacity, the total antioxidant capacity (4.34 mM) and T-SOD activity (158.22 U/mgprot) of the decolored peptide group were significantly better than those of other treatment groups (p < 0.05). Iron metabolism results showed that TIBC value (127.4 μmol/L) of the composite peptide group was not significantly different from that of the normal group (p > 0.05), indicating its superior ability to regulate iron homeostasis. The hepcidin levels of the experimental groups (including the decolored peptide group, non-decolored peptide group, and composite peptide group) were not significantly different from those of the normal group (p > 0.05), suggesting that the experimental groups could restore hepcidin to normal physiological levels, with the decolored peptide group showing the most significant effect. Conclusion Tuna Dark Muscle-Derived Peptide Products exhibit superior therapeutic effects to traditional ferrous sulfate supplements and positive controls in improving IDA, with decolorized peptide demonstrating the best results. These findings provide a theoretical basis for the development of novel peptide-based iron supplements. |
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