吴家敏,仲伟茂,车逸豪,等.南海冷泉来源真菌Talaromyces verruculosus CYH030次级代谢产物及其α-葡萄糖苷酶抑制活性研究[J].中国海洋药物,2026,(4):-.
南海冷泉来源真菌Talaromyces verruculosus CYH030次级代谢产物及其α-葡萄糖苷酶抑制活性研究
Study on the Secondary Metabolites and Their α-Glucosidase Inhibitory Activity of a South China Sea Cold Seep-derived Fungus Talaromyces verruculosus CYH030
投稿时间：2025-03-05  修订日期：2025-04-30
DOI：
中文关键词:  冷泉  篮状菌Talaromyces verruculosus  次级代谢产物  α-葡萄糖苷酶抑制活性
English Keywords:Cold seep  Talaromyces verruculosus  secondary metabolites  α-glucosidase inhibitory activity
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作者单位邮编
吴家敏 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室
中国科学院大学 		510301
仲伟茂 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室
中国科学院大学 
车逸豪 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室；大理大学 
周子义 广州中医药大学青蒿研究中心 
王发左* 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室 510301
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中文摘要:
      目的 对一株中国南海冷泉来源真菌Talaromyces verruculosus CYH030次级代谢产物及其α-葡萄糖苷酶抑制活性进行研究。方法 通过条件优化,选择最合适发酵培养条件对菌株CYH030进行小规模化发酵,采用硅胶柱层析、十八烷基硅烷键合反相柱层析(ODS)和Sephadex LH-20凝胶柱层析以及高效液相色谱(HPLC)等分离手段对菌株CYH030在沙氏葡萄糖培养基(SDB)中的发酵产物进行分离纯化,利用核磁共振波谱(NMR)、低分辨电喷雾质谱(ESI-MS)和X射线单晶衍射技术,结合文献数据对比鉴定化合物的化学结构,并对化合物进行α-葡萄糖苷酶抑制活性测试。结果 从篮状菌Talaromyces verruculosus CYH030的发酵产物中分离鉴定18个单体化合物,其中化合物1和2为新天然产物,化合物3为首次从Talaromyces属真菌中发现。α-葡萄糖苷酶抑制活性结果表明,化合物显现出不同程度的α-葡萄糖苷酶抑制活性。
English Summary:
      Objective To study the secondary metabolites and their α-glucosidase inhibitory activity of a South China Sea cold seep-derived fungus Talaromyces verruculosus CYH030. Methods The optimized fermentation condition was selected for a scale-up fermentation of the strain CYH030. The fermentation crude extract of the strain CYH030 in Sabouraud dextrose broth (SDB) medium was separated and purified by silica gel column chromatography, octadecylsilane bonded silica gel column chromatography (ODS) and Sephadex LH-20 gel column chromatography as well as high-performance liquid chromatography (HPLC). Structures of these compounds were elucidated by nuclear magnetic resonance spectroscopy (NMR), electrospray ionization mass spectrometry (ESI-MS), X-ray single crystal diffraction, as well as data comparisons with those from literatures. These isolates were evaluated for α-glucosidase inhibitory activity. Results Eighteen compounds were isolated from the fungal strain Talaromyces verruculosus CYH030. Compounds 1 and 2 were new natural products, and compound 3 was isolated for the first time from Talaromyces fungus. All compounds exhibited different degrees of α-glucosidase inhibitory in their bioactivity evaluation.
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