| 韩旭,刘明利,姜廷福,等.在线扫集-胶束毛细管电动色谱法测定海葵中核苷类化合物含量[J].中国海洋药物,2026,(1):-. |
| 在线扫集-胶束毛细管电动色谱法测定海葵中核苷类化合物含量 |
| Determination of Nucleosides in Sea Anemones by Sweeping-Micellar Electrokinetic Chromatography |
| 投稿时间：2024-10-10 修订日期：2024-11-21 |
| DOI： |
| 中文关键词: 扫集-胶束毛细管电动色谱 核苷 海葵 |
| English Keywords:sweeping-micellar electrokinetic chromatography nucleosides sea anemones |
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| 中文摘要: |
| 目的 采用在线扫集-胶束毛细管电动色谱法（Sweeping-MEKC）测定海葵中10种常见核苷类化合物含量。方法 最佳毛细管电泳条件为：缓冲液为40 mmol/L十二烷基硫酸钠（SDS）-10%甲醇-15 mmol/L硼砂（pH = 9.2），检测波长200 nm，进样压力5 000 Pa，进样时间80 s，运行电压25 kV，运行温度25 ℃。结果 10种核苷类化合物显示出良好的线性关系（r≥0.999 4），最低检测限为0.20～0.50 μg/mL，且富集效果显著。该方法成功检测出海葵中含有2’-脱氧鸟苷、次黄嘌呤、鸟苷、尿嘧啶核苷、肌苷、黄嘌呤6种核苷类成分，加标回收率在93.53%～106.25%之间，且相对标准偏差（RSD）不超过4.6%（n = 3）。结论 该方法简单高效，富集效果与重复性良好，成功测定了海葵中核苷类化合物的含量，为毛细管电泳技术高灵敏地检测多种核苷类化合物提供了研究基础。 |
| English Summary: |
| Objective To determine the content of 10 common nucleosides in sea anemones using sweeping-micellar electrokinetic chromatography (Sweeping-MEKC). Methods The optimal electrophoretic conditions were as follows: buffer consisting of 40 mmol/L sodium dodecyl sulfate (SDS)-10% methanol-15 mmol/L borate (pH = 9.2), detection wavelength of 200 nm, injection pressure of 5 000 Pa, injection time of 80 seconds, running voltage of 25 kV and running temperature of 25 ℃. Results The 10 nucleosides showed good linearity (r≥0.999 4), with a minimum detection limit of 0.20 to 0.50 μg/mL, and the enrichment effect was significant. The method successfully detected 6 nucleosides in the sea anemone, including 2’-deoxyguanosine, xanthine, guanosine, uridine, inosine, and hypoxanthine. The spiked recovery rate was between 93.53% and 106.25%, and the relative standard deviation (RSD) did not exceed 4.6% (n = 3). Conclusion This method is simple, efficient, and has good enrichment and repeatability, and has successfully determined the content of nucleosides in sea anemone. It provides a foundation for the highly sensitive detection of various nucleosides by capillary electrophoresis technology. |
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