| 丁金铭,朱阳东,于斋卓,等.响应面法优化酶法提取羊栖菜多糖工艺及体外降血糖活性研究[J].中国海洋药物,2026,(1):-. |
| 响应面法优化酶法提取羊栖菜多糖工艺及体外降血糖活性研究 |
| Optimization of enzymatic extraction process of polysaccharide from Sargassum fusiforme by response surface method and its hypoglycemic activity in vitro |
| 投稿时间：2024-08-20 修订日期：2024-11-07 |
| DOI： |
| 中文关键词: 羊栖菜多糖 纤维素酶 响应面法 体外降血糖 |
| English Keywords:Sargassum fusiforme polysaccharide cellulase response surface methodology In vitro hypoglycemia |
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| 中文摘要: |
| 目的：优化酶法提取羊栖菜多糖的工艺。方法 以羊栖菜为原料，以酶解时间、纤维素酶添加量、料液质量浓度和酶解温度为单因素，分析单因素对羊栖菜多糖得率的影响，通过响应面法优化羊栖菜多糖的提取工艺，利用高效液相色谱技术测定单糖组成，采用体外抑制α-葡萄糖苷酶和α-淀粉酶的活性实验对其降血糖活性进行初步研究。结果 羊栖菜多糖的最佳提取条件为：酶解时间136 min、纤维素酶添加量56（U/g）、料液质量浓度0.05（g/mL）、酶解温度46 ℃。在此条件下，羊栖菜多糖的得率达到（14.20 ± 0.13）%。该方法提取的羊栖菜多糖由甘露糖醛酸、古罗糖醛酸、岩藻糖和半乳糖等单糖组成，羊栖菜多糖对α-葡萄糖苷酶和α-淀粉酶具有较好的抑制活性，其半抑制浓度分别为0.487、2.073 mg/mL。结论 得到的优化酶法提取工艺可以有效提取羊栖菜多糖，且提取的羊栖菜多糖具有较好的体外降血糖活性，可为羊栖菜多糖在降血糖药物开发中的潜在应用提供依据 |
| English Summary: |
| Objective To optimize the enzymatic extraction of polysaccharides from Sargassum fusiforme. Methods Enzymatic hydrolysis time, cellulase addition amount, mass concentration of material solution and enzymatic hydrolysis temperature were used as single factors to analyze the influence of single factors on the yield of polysaccharide from Sargassum fusiforme. The extraction process of Sargassum fusiforme. polysaccharide was optimized by response surface method,and the composition of monosaccharides was determined by high performance liquid chromatography. The in vitro inhibition of α-glucosidase and α-amylase activity was used to study the hypoglycemic activity. Results The optimum extraction conditions were as following: enzymolysis time 136 min, cellulase amount 56 (U/g), mass concentration of material solution 0.05 (g/mL), enzymolysis temperature 46 ℃. Under these conditions, the yield of polysaccharide was (14.20 ± 0.13)%. The polysaccharide extracted by this method was composed of monosaccharides such as mannuronic acid, gururonic acid, fucose and galactose. The extracted polysaccharide had a good inhibitory activity on α-glucosidase and α-amylase, and the semi-inhibitory concentration was 0.487 and 2.073 mg/mL, respectively. Conclusions The optimized enzymatic extraction process can effectively extract the polysaccharide from Sargassum fusiforme, and the extracted polysaccharide has a good hypoglycemic activity in vitro, which provides a basis for the potential application of Sargassum fusiforme polysaccharide in the development of hypoglycemic drugs. |
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