| 黄晓雯,张宇璠,杨 斌,等.红树林来源Kosakonia sp. SCSIO 43811的次级代谢产物研究[J].中国海洋药物,2025,(5):-. |
| 红树林来源Kosakonia sp. SCSIO 43811的次级代谢产物研究 |
| Study on Secondary Metabolites of Kosakonia sp., a Source of Mangrove Forests |
| 投稿时间：2024-07-09 修订日期：2024-09-20 |
| DOI： |
| 中文关键词: 红树林 Kosakonia sp. 次级代谢产物 结构鉴定 乙酰胆碱酯抑制活性 |
| English Keywords:mangroves Kosakonia sp Secondary metabolites Structural identification Acetylcholine ester inhibitory activity |
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| 中文摘要: |
| 目的 本研究基于OSMAC策略对一株广西南海红树林来源的Kosakonia sp.次级代谢产物中结构新颖、活性优良的化合物进行挖掘和探索。方法 采用多种色谱技术方法对该菌的次级代谢产物进行分离纯化,并运用NMR、MS以及与相关文献数据比对方法进行化合物结构鉴定,并对化合物进行乙酰胆碱酯酶抑制活性和抗菌活性相关评价。结果 从红树林来源Kosakonia sp. SCSIO 43811得到11个已知化合物,其中8个环肽类,即cyclo (L-Trp-D-Val)(1)、cyclo (Trp-Val)(2)、cyclo (L-Trp-L-Phe)(3)、cis-cyclo (Pro-Trp)(4)、cyclo (Phe-Val)(5)、cyclo (Phe-Val)(6)、cyclo (L-Pro-L-Leu)(7)、cyclo (L-Phe-L-Pro)(8)；1个咔啉衍生物,即1-acetyl-β-carboline(9);两个吲哚衍生物,3,3-二-(3-吲哚)丙烷-1,2-二醇(10),即2-(1H-indol-3-yl) ethyl acetate(11)。化合物1-11均是首次红树林来源的卡萨克尼亚氏菌属(Kosakonia sp.)中分离获得。活性测试结果显示,化合物1,4,6,7,8,9,11对乙酰胆碱酯酶有一定的抑制活性。本研究丰富了红树林来源的卡萨克尼亚氏菌属(Kosakonia sp.)次级代谢产物多样性。 |
| English Summary: |
| Objective This study is based on the OSMAC strategy to explore compounds with novel structures and excellent activities in the secondary metabolites of Kosakonia sp. derived from mangrove forests in the South China Sea of Guangxi province. Methods Multiple chromatographic techniques were used to isolate and purify the secondary metabolites of this bacterium, and NMR, MS, and comparison with relevant literature data were used to identify the structure of compounds. The enzymatic and antibacterial activities of the products were evaluated by using the Kirby-Bauer test and the acetylcholinesterase radical scavenging method. Results, 11 known compounds were obtained from Kosakonia sp, including 8 cyclic peptides, namely cyclo (L-Trp-D-Val) (1), cyclo (Trp-Val) (2), cyclo (L-Trp-L-Phe) (3), cis cyclo (Pro-Trp) (4), cyclo (Phe-Val) (5), cyclo (Phe-Val) (6), cyclo (L-Pro-L-Leu) (7), and cyclo (L-Phe-L-Pro) (8); 1 carbaline derivative, 1- acetyl- β- Caroline (9); Two indole derivatives, namely 3,3- dis- (3-indole) propane- 1,2- diol (10), 2- (1H indol-3-yl) ethyl acetate (11). Compounds 1-11 were isolated for the first time from the genus Kosakonia sp. derived from mangrove forests. Compounds 1, 4, 6, 7, 8, 9 and 11 showed certain inhibitory activity against acetylcholinesterase; This study enriched the diversity of secondary metabolites of Kosakonia sp. from mangrove sources. |
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