| 乔鑫,韩璐.复合氧化铈纳米酶水凝胶微针的制备及表征[J].中国海洋药物,2026,45(1):36-44. |
| 复合氧化铈纳米酶水凝胶微针的制备及表征 |
| Preparation and characterization of cerium oxide nano-enzyme composited microneedle |
| 投稿时间：2024-04-20 修订日期：2024-07-26 |
| DOI：10.13400/j.cnki.cjmd.2026.01.008 |
| 中文关键词: 仿贻贝 氧化铈 介孔硅 水凝胶微针 |
| English Keywords:polydopamine Cerium oxide Mesoporous silica nanoparticle microneedles |
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| 中文摘要: |
| 目的 制备表面有氧化铈的核壳介孔二氧化硅纳米颗粒(PCeO2-MSN),同时考察其形貌结构和体外抗氧化性能。以聚乙烯醇(PVA)和聚乙烯吡咯烷酮K30(PVP K30)为基底,以甲基丙烯酸化透明质酸(HAMA)为针尖,并将核壳纳米颗粒PCeO2-MSN复合到微针针尖中,制备复合氧化铈纳米酶水凝胶微针。考察微针的力学性能以及溶胀效果。方法 首先,使用改良的Stober法合成了介孔二氧化硅纳米颗粒,随后采用仿贻贝多巴胺介导合成策略,在介孔二氧化硅纳米颗粒表面原位生长具有抗氧化性能的氧化铈纳米颗粒,制备核壳纳米颗粒PCeO2-MSN。采用透射电镜考察纳米颗粒形貌以及采用X射线衍射仪对纳米颗粒进行了物相分析,同时采用xx方式考察纳米颗粒的抗氧化性能。其次,以透明质酸和甲基丙烯酸酐为原料合成HAMA,将纳米颗粒与HAMA前体溶液混合,PVA与PVP K30 混合得到基底溶液,采用两步离心法,37℃干燥制备微针,通过对微针进行形貌观察,压缩实验、刺入实验以及溶胀实验,考察微针的力学性能以及刺入性能。结论 基于仿贻贝多巴胺优异的金属离子螯和能力,成功制备了具有羟基自由基的清除活性的PCeO2-MSN,该纳米颗粒分散性良好,XRD结果显示原位生长的氧化铈保持萤石晶型结构；并成功制备了复合PCeO2-MSN的HAMA微针,微针形貌良好,力学性能为每针2.0 N, 且能成功刺入皮肤,有望用于经皮纳米酶递送,从而治疗氧化应激引发的皮肤疾病。 |
| English Summary: |
| Aim Core-shell mesoporous silica nanoparticles ( PCeO2-MSN ) with cerium oxide on the surface were prepared and their antioxidant property was evaluated. Then PCeO2-MSN composited hydrogel microneedle was prepared by using polyvinyl alcohol (PVA) and polyvinylpyrrolidone K30 (PVP K30) as the matrix of substrate, incorporating PCeO2-MSN in the methacrylated hyaluronic acid (HAMA) based the matrix of tip of the needle. The mechanical properties of the microneedle as well as the dissolution effect were investigated. Methods Mesoporous silica nanoparticles (MSN) were firstly synthesized based on a modified Stober method. Then the cerium oxide nanoparticles were in situ synthesized on the surface of MSN based on polydopamine mediated method to obtain PCeO2-MSN. The morphology of the PCeO2-MSN was investigated by transmission electron microscope and the physical phase was analyzed by X-ray diffractometer. The in vitro antioxidant property of the PCeO2-MSN were also evaluated by xx. For fabrication of microneedle, HAMA was firstly synthesized from hyaluronic acid and methacrylic anhydride. Then the PCeO2-MSN were mixed with HAMA precursor solution, and PVA was mixed with PVP K30 to obtain the substrate solution, and the microneedles were prepared by two-step centrifugation and dried at 37℃, and the mechanical and puncture properties of microneedles were investigated through the observation of microneedles" morphology, and compression, puncture, and dissolution experiments. Results The PCeO2-MSN with excellent hydroxyl radical scavenging activity was successfully synthesized. The PCeO2-MSN composited microneedles were also prepared with the mechanical strength of 2.0 N per needle, which can successfully penetrated into the skin. |
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