| 许蕙馨,蔡凯宇,李燕楠,等.藻酸双酯钠对环磷酰胺所致小鼠免疫抑制的调节作用[J].中国海洋药物,2025,(6):-. |
| 藻酸双酯钠对环磷酰胺所致小鼠免疫抑制的调节作用 |
| Immunity of PSS to cyclophosphamide mice |
| 投稿时间：2024-03-26 修订日期：2024-06-13 |
| DOI： |
| 中文关键词: 藻酸双酯钠、环磷酰胺、免疫调节、毒性、药代 |
| English Keywords:cyclophosphamide, immunity, immune deficiency, polysaccharide |
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| 中文摘要: |
| 目的 研究藻酸双酯钠(Propylene glycol alginate sodium sulfate,PSS)对小鼠免疫功能的影响。方法 将C57BL/6J小鼠随机分成4组,1组为正常小鼠,3组为环磷酰胺(CTX)诱导的免疫抑制小鼠。CTX诱导组每日分别以0、30 mg/kg(PSS)、1100IU(LWMH)剂量腹腔注射给药,持续14 d,测定小鼠血常规指标、胸腺指数和脾指数。采用急慢性毒性,分析PSS的安全性。采用小动物活体光学成像系统分析PSS的体内分布情况。采用血凝仪-APTT法分析PSS的初步药代动力学。结果 给予PSS的免疫抑制小鼠血液WBC、Lymph、NEUT、MONO、RBC、PLT、HGB水平显著提高；PSS对正常小鼠有明显的免疫调节作用,并能以剂量依赖的方式增强小鼠的免疫状态,没有明显毒性；PSS进入体内15 min左右主要集中到肝脏组织中进行代谢,60 min后基本代谢完全；尾静脉给药的血浆中药物最高浓度大于腹腔注射、灌胃给药,腹腔注射血浆中药物消除最慢。结论 PSS能显著增强免疫抑制小鼠和正常小鼠的免疫功能。 |
| English Summary: |
| Objective To study the effects of Propylene alginate sodium sulfate (PSS) on the immune function of mice. Methods C57 mice were randomly divided into 4 groups, group 1 was normal mice, group 3 was CTX-induced immunosuppressive mice. In the CTX-induced group, the mice were injected intraperitoneally at the dose of 0, 30 mg/kg (PSS) and 1100IU (LWMH), respectively, for 14 days, and the blood routine indexes, thymus index and spleen index were determined. Acute and chronic toxicity was used to analyze the safety of PSS. The distribution of PSS in vivo was analyzed by in vivo optical imaging system of small animals. The primary pharmacokinetics of PSS were analyzed by APTT method. Results The blood WBC, Lymph, NEUT, MONO, RBC, PLT and HGB levels of immunosuppressed mice were significantly increased after PSS treatment. PSS has obvious immunomodulatory effect on normal mice, and can enhance the immune status of mice in a dose-dependent way, without obvious toxicity. PSS was mainly concentrated in liver tissue for metabolism about 15 min after entering the body, and the metabolism was basically complete after 60 min. The maximum concentration of drugs in plasma given by caudal vein was higher than that given by intraperitoneal injection and intragastric administration, and the elimination of drugs in plasma by intraperitoneal injection was the slowest. Conclusion PSS can significantly enhance the immune function of immunosuppressed mice and normal mice. |
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