| 王文君,王丹妮,张雨竹,等.来自海洋细菌 Thalassomonas sp. LD5 的 PL25 家族石莼多糖裂解酶的生化特征[J].中国海洋药物,2025,(3):-. |
| 来自海洋细菌 Thalassomonas sp. LD5 的 PL25 家族石莼多糖裂解酶的生化特征 |
| Biochemical Characterization of a PL25 Family Ulvan Lyase from Marine Bacterium Thalassomonas sp. LD5 |
| 投稿时间：2024-02-28 修订日期：2024-03-25 |
| DOI： |
| 中文关键词: 石莼 石莼多糖裂解酶 石莼寡糖 多糖裂解酶25家族。 |
| English Keywords:ulvan ulvan lyase ulvan oligosaccharides polysaccharide lyase family 25. |
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| 中文摘要: |
| 目的：克隆和表达一种新的 PL25 家族石莼多糖裂解酶 TsUly25A，研究其特性并探索其在制备石莼寡糖中的应用。
方法： 从 Thalassomonas sp. LD5 中克隆出编码 PL25 家族石莼多糖裂解酶的基因 tsuly25A，并在大肠杆菌 BL21 (DE3) 中进行表达。随后纯化了重组酶 TsUly25A，并对其酶学性质进行了研究。
结果：TsUly25A 在 50 °C、pH 8.0 条件下酶活性最高，比活力为 0.45 U/mg。NaCl对 TsUly25A 的活性和热稳定性影响甚微。值得注意的是，TsUly25A 在纯水中仍能降解石莼多糖，并保留了约 60% 的活性。
结论：石莼多糖裂解酶TsUly25A能在纯水中降解石莼多糖，为石莼多糖裂解酶在工业上应用于生产石莼寡糖奠定了基础。 |
| English Summary: |
| Aims: To clone and express a new PL25 family ulvan lyase, TsUly25A, and to characterise its properties and explore its application for the preparation of ulvan oligosaccharides.
Methods: The gene encoding ulvan lyase of the PL25 family, tsuly25A, was cloned from Thalassomonas sp. LD5 and then expressed in Escherichia coli BL21 (DE3). The recombinant enzyme TsUly25A was subsequently purified and its enzymatic properties were investigated.
Results: Its enzyme activity was highest at 50 °C and pH 8.0, with a specific activity of 0.45 U/mg. NaCl exerted marginal effects on the activity and thermal stability of TsUly25A. Noteworthily, TsUly25A could still degrade ulvan polysaccharides in pure water and retained about 60% of its activity.
Conclusions: Ulvan lyase TsUly25A can degrade ulvan polysaccharides in pure water, laying the foundation for the industrial application of ulvan lyase in the production of ulvan oligosaccharides. |
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