| 仲天毅,唐伟卓.基于表观遗传修饰的海绵真菌Aspergillus tritici化学多样性及代谢产物研究[J].中国海洋药物,2025,(5):-. |
| 基于表观遗传修饰的海绵真菌Aspergillus tritici化学多样性及代谢产物研究 |
| Zhong Tian-Yi, Jiang Lin-na, Xia Die, Tan Yi, He Jia-lin, TANG Wei-zhuo* |
| 投稿时间：2024-02-22 修订日期：2024-08-07 |
| DOI： |
| 中文关键词: 表观遗传修饰 海绵真菌 化学多样性 次级代谢产物 抗植物病菌活性 |
| English Keywords:Epigenetics modification, sponge-derive fungus, chemical diversity, secondary metabolites, anti-plant pathogens activity |
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| 中文摘要: |
| 本文首先考察两种表观遗传试剂丙戊酸钠(VPA)和5-氮杂胞苷(5-zazC)调控海绵来源真菌Aspergillus tritici化学多样性的影响，筛选出最佳培养条件。随后运用硅胶柱层析、ODS和半制备液相等色谱手段对最优培养条件下的菌株发酵物开展次级代谢产物研究，并通过MS、NMR等光谱技术鉴定结构。最后，运用K-B法和LPS诱导RAW264.7细胞炎症模型评价分离化合物的抗菌和抗炎活性。结果显示，菌株培养基中加入5-zazC后经大米固体发酵，化学多样性显著增强，其代谢产物在原有1个主产物的基础上，新产生4个化合物。进一步通过分离纯化，上述化合物分别鉴定为1,4-dimethoxy-2,4',4"-trihydroxy-p-terphenyl (1)，cyclo (D)-Pro-(D)-Leu (2)， NP-101A (3)，phenylacetamide (4)和 N-phenethylacetamide (5)，上述化合物均为首次从该属真菌中分离。化合物1显示了中等的抗植物病菌和抑制NO释放活性。 |
| English Summary: |
| In this present study, the chemical diversity of an sponge-derived fungus Aspergillus tritici was investigated by using epigenetic manipulation regulation strategy. Further, the secondary metabolites from the A. tritici was isolated based on silica, ODS column chromatography, and semi-preparative chromatograhpy, and their structures were assigned by interperation of 1D, and 2D NMR spectrocsopic, and MS data. Finally, the anti-plant pathogens and anti-inflammatory activity of the isolated compounds was evaluated. The results showed that the chemical diversity of the strain was significantly enhanced after the addition of 5-zazC to the strain medium, and four newly metabolites were produced under the solid fermentation. Further, these five compounds, namely, 1,4-dimethoxy-2,4',4"-trihydroxy-p-terphenyl (1),cyclo (D)-Pro-(D)-Leu (2), NP-101A (3),phenylacetamide (4), and N-phenethylacetamide (5) were identified from the A. tritici and these metabolites were firstly reported from the genus Aspergillus. The biological evaluation results shown that compound 1 had moderate inhibitory activity against plant pathogens and NO production. |
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