| 屈瑞敬,王倩倩,顾玉超.动态控制胞内碳通量促进大肠杆菌高效合成肌醇[J].中国海洋药物,2025,(1):-. |
| 动态控制胞内碳通量促进大肠杆菌高效合成肌醇 |
| Dynamic control of intracellular carbon flux promotes efficient inositol synthesis by E. coli |
| 投稿时间：2023-12-13 修订日期：2023-12-26 |
| DOI： |
| 中文关键词: 大肠杆菌 肌醇 动态控制 |
| English Keywords:E. coli Inositol Dynamic control |
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| 中文摘要: |
| 肌醇,是细胞生命活动的必需物质,也是许多药物前体,主要通过植酸水解法得到,工艺繁琐且原料利用率低。本文在大肠杆菌MG1655(DE3)中异源表达肌醇-1-磷酸合酶(INO1),构建了肌醇合成途径,利用CRISPR-Cas9技术构建的质粒消除系统,对糖酵解途径(EMP)关键酶果糖-6-磷酸激酶和糖摄取途径磷酸烯醇式丙酮酸(PEP)-磷酸转移酶系统(PTS系统)进行动态控制,提高碳源利用率,减少代谢分流,最终,通过摇瓶发酵得到肌醇产量218.03 mg/L。 |
| English Summary: |
| Inositol, an essential substance for cell life activities, is also the precursor of many drugs. It is mainly obtained by phytic acid hydrolysis. The process is complicated and the utilization rate of raw materials is low. In this paper, inositol-1-phosphate synthase (INO1) was heterologous expressed in Escherichia coli MG1655(DE3), inositol synthesis pathway was established, and plasmid elimination system was constructed using CRISPR-Cas9 technology. The key enzymes of glycolysis pathway fructose-6-phosphokinase and sugar uptake pathway phosphoenolpyruvate (PEP) -phosphotransferase system (PTS system) were dynamically controlled to improve the carbon source utilization and reduce metabolic diversion. Finally, the inositol yield was 218.03 mg/L by shaker fermentation. |
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