| 吴萍萍,迟茜文,黄薇,等.海洋本草软珊瑚共附生真菌Aspergillus versicolor CGF9-1-2化学成分研究[J].中国海洋药物,2025,(1):-. |
| 海洋本草软珊瑚共附生真菌Aspergillus versicolor CGF9-1-2化学成分研究 |
| Research on the Secondary Metabolites from Soft Coral-associated Fungus Aspergillus versicolor CGF9-1-2 |
| 投稿时间：2023-11-02 修订日期：2023-12-29 |
| DOI： |
| 中文关键词: 海洋本草软珊瑚 Aspergillus versicolor 生物碱 酚酸 结构鉴定 |
| English Keywords:Marine herb soft coral-associated Aspergillus versicolor alkaloid phenolic acids identification |
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| 中文摘要: |
| 摘 要：目的 对一株软珊瑚来源共附生真菌Aspergillus versicolor CGF9-1-2的次生代谢产物进行研究。方法 采用硅胶柱层析、ODS柱层析、HPLC等色谱分离方法对其代谢产物进行分离纯化，运用HR-ESI-MS、NMR、ORD等技术及对分离得到的化合物进行结构鉴定，并对其进行TDP1活性测试。结果 从Aspergillus versicolor CGF9-1-2中分离得到10个化合物，分别鉴定为(2S, 2'R, 3R, 3'E, 4E, 8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2'-hydroxyl-3'-octadecenoyl)amino-9-methyl-4,8-octadecadiene(1)、(4E, 8E)-N-2'-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine (2)、desoxybrevianamide E (3)、cyclo-(D-Pro-D-Phe) (4)、cyclo-(L-Pro-D-Leu) (5)、indole-3-carboxy-acid (6)、β-Carboline (7)、(7S)-(+)-Hydroxysydonic acid (8)、ent-Aspergoterpenin C (9)和(E)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (10)。所有化合物对TDP1均无明显抑制活性。结论 化合物2为首次从Aspergillus属真菌分离得到，化合物1为首次从Aspergillus versicolor真菌分离得到。 |
| English Summary: |
| Abstract: Objective To study the chemical structures of secondary metabolites from soft coral derived fungus Aspergillus versicolor CGF9-1-2. Methods The secondary metabolites were separated and purified by silica gel column chromatography, ODS column chromatography, Sephadex LH-20 column chromatography, and HPLC. The structures of isolated compounds were subjected to high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR), and optical rotation dispersion (ORD) spectroscopy. They were also tested for their TDP1 activity. Results From the culture of Aspergillus versicolor CGF9-1-2, we successfully isolated and identified two sphingoglycolipid compounds(2S, 2'R, 3R, 3'E, 4E, 8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2'-hydroxyl-3'-octadecenoyl)amino-9-methyl-4,8-octadecadiene (1), (4E, 8E)-N-2'-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine (2), five alkaloids: desoxybrevianamide E (3), cyclo-(D-Pro-D-Phe) (4), cyclo-(L-Pro-D-Leu) (5), indole-3-carboxylic-acid (6), and β-Carboline (7). In addition, three simple phenolic acid compounds (7S)-(+)-Hydroxysydonic acid (8), ent-Aspergoterpenin C (9) and (E)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (10). None of these compounds showed significant TDP1 inhibitory activity. Conclusion 2 was firstly isolated from Aspergillus, and 1 was obtained from Aspergillus versicolor for the first time. |
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