| 王梦芹,帖青清,黄晓雯,等.南海珊瑚共附生真菌Nodulisporium sp. BH 2-5的次级代谢产物及活性研究[J].中国海洋药物,2025,44(3):45-52. |
| 南海珊瑚共附生真菌Nodulisporium sp. BH 2-5的次级代谢产物及活性研究 |
| Study on Secondary Metabolites and Activities of Nodulisporium sp. BH 2-5, a Coral Coexisting Fungus from the South China Sea |
| 投稿时间：2023-11-16 修订日期：2024-01-02 |
| DOI：10.13400/j.cnki.cjmd.2025.03.008 |
| 中文关键词: Nodulisporium sp. 次级代谢产物 酶抑制活性 抗菌活性 |
| English Keywords:Nodulisporium sp Secondary metabolites enzyme inhibitory activity antibacterial activity |
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| 中文摘要: |
| 目的 对一株海洋真菌Nodulisporium sp. BH 2-5的次级代谢产物中结构新颖、活性优良的化合物进行挖掘和探索。方法 采用多种色谱技术方法对该菌的次级代谢产物进行分离纯化,并运用NMR、MS以及与相关文献数据比对方法进行化合物结构鉴定。进行乙酰胆碱酯酶(AchE),胰脂肪酶(PL),神经氨酸酶(NA)抑制活性和抗菌活性的评价。结果 从海洋真菌Nodulisporium sp. BH 2-5中分离得到了13个已知聚酮类化合物,分别是2-(2-羟基乙基)苯酚(1)、1-(2,6-二羟基苯基)-3- 羟基丁-1- 酮(2)、1-(2,6-二羟基苯)丁-1- 酮(3)、1- 丁基间苯三酚(4)、2- 甲基间苯二酚(5)、2-N- 丙基 -5,7- 二羟基色酮(6)、(2"S)-2-(丙醇)-5- 羟基 - 苯并吡喃 -4- 酮(7)、(R) -2,3- 二氢-5- 羟基 -2- 甲基色烯 -4- 酮(8)、(2S)-2,3- 二氢 -5,6- 二羟基 -2- 甲基 -4H-1- 苯并吡喃 -4- 酮(9)、5,8- 二羟基 -2- 甲基 -4H-1- 苯并吡喃 -4- 酮(10)、(2R,4S)-2,3- 二氢-2- 甲基 - 苯并吡喃 - 4,5- 二醇(11)、(2R,4R)-3,4- 二氢 -4- 甲氧基 - 2 - 甲基 -2H-1- 苯并吡喃 -5- 醇(12)、Aspergillusphenol D(13)。结论 化合物13为首次从多节孢属真菌次级代谢产物中分离得到。化合物4和10对乙酰胆碱酯酶有明显的抑制作用；化合物5、7、10、11、12对胰脂肪酶有微弱的活性；化合物1-10对神经氨酸酶有明显抑制效果；化合物13有明显的抗菌活性。 |
| English Summary: |
| Objective To explore and explore compounds with novel structures and excellent activity in the secondary metabolites of a marine fungus Nodulisporium sp. BH 2-5. Methods Multiple chromatographic techniques were used to isolate and purify the secondary metabolites of this bacterium, and NMR, MS, and comparison with relevant literature data were used to identify the compound structure. Evaluate the antibacterial activities and inhibitory activities of acetylcholinesterase (AchE), pancreatic lipase (PL), and neuraminidase (NA). Results Thirteen known polyketide compounds were isolated from marine fungus Nodulisporium sp. BH 2-5, named 2- (2-hydroxyethyl)phenol (1), 1- (2,6-dihydroxyphenyl)-3-hydroxybutan-1-one (2), 1- (2,6-dihydroxyphenyl)butan-1-one (3), 1-butyrylphloroglucinol (4), 2-Methylresorcinol (5), 2-N-Propyl-5,7-dihydroxychromone (6), (2’ S)-2- (propan-20 -ol)-5-hydroxy-benzopyran-4-one (7), (R)-2,3-dihydro-5-hydroxy-2-methylchromen-4-one (8), (2S)-2,3-dihydro-5,6-dihydroxy-2-methyl-4H-1-benzopyran- 4-one (9), 5,8-Dihydroxy-2-methyl-4H-1-benzopyran-4-one (10), (2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol (11), (2R, 4R) -3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol (12),, Aspergillusphenol D (13). Conclusion Compound 13 was isolated for the first time from the fungus Nodulisporium sp. Compounds 4 and 10 have a significant inhibitory effect on acetylcholinesterase; Compounds 5, 7, 10, 11, and 12 have weak activity against pancreatic lipase; Compounds 1-10 have a significant inhibitory effect on neuraminidase; Compound 13 has significant antibacterial activity. |
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