| 廖海金,陈旭珂,王文静,等.海洋细菌Bacillus sp. T1-7B次级代谢产物及其抑菌活性研究[J].中国海洋药物,2025,44(3):60-66. |
| 海洋细菌Bacillus sp. T1-7B次级代谢产物及其抑菌活性研究 |
| Secondary metabolites from Bacillus sp. T1-7B and their antibacterial activity |
| 投稿时间：2023-10-27 修订日期：2023-12-05 |
| DOI：10.13400/j.cnki.cjmd.2025.03.006 |
| 中文关键词: 海洋细菌 次级代谢产物 环二肽 抑菌 |
| English Keywords:marine bacteria secondary metabolites cyclodipeptide antibacterial |
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| 中文摘要: |
| 目的 研究舟山海域潮间带（122°15′39″ E，30°4′31″ N）沉积泥中分离得到的芽孢杆菌Bacillus sp. T1-7B的次级代谢产物及其抑菌活性。方法 对Bacillus sp. T1-7B的发酵产物采用乙酸乙酯和正丁醇萃取，获得的萃取物经常压硅胶柱色谱、Sephadex LH-20凝胶柱色谱及HPLC等分离纯化，结合核磁共振光谱数据并参考文献数据鉴定化合物的结构；通过纸片法和稀释法对各化合物的抑菌活性进行测定。结果 从Bacillus sp. T1-7B的发酵产物获得9个环二肽类化合物和1个二肽类化合物，其结构分别为环[(S)-脯氨酸-(R)-亮氨酸]（T-1）、(S)-脯氨酰胺-(R)-缬氨酸二肽（T-2）、环[(S)-亮氨酸-(S)缬氨酸]二肽（T-3）、callyspongidipeptide A（T-4）、环-[(S)-脯氨酸-(R)-苯丙氨酸]（T-5）、环[(S)-脯氨酸-(S)-亮氨酸]（T-6）、环[(S)-脯氨酸-(S)-缬氨酸]（T-7）、环[(S)-脯氨酸-(S)-异亮氨酸]（T-8）、环[(S)-脯氨酸-(S)-丙氨酸]二肽（T-9）和环-(苯丙氨酸-丝氨酸)（T-10）。化合物T-5、T-7和T-8对金黄色葡萄球菌的最低抑制浓度（MIC）分别为8、4和8 μg/mL。结论 化合物T-2、T-4和T-10为首次从该属中分离得到。化合物T-5、T-7和T-8对金黄色葡萄球菌有较为明显的抑制效果。 |
| English Summary: |
| Objective To investigate the antibacterial secondary metabolites of Bacillus sp. T1-7B isolated and purified from the sediment of Zhoushan intertidal zone (122°15′39″ E, 30°4′31″ N). Method The ethyl acetate and n-butanol extracts of the fermentation broth were isolated and purified by silica gel column chromatography, sephadex LH-20 gel chromatography and HPLC. The structures of secondary metabolites were identified by NMR spectroscopic data and reference data. Antibacterial activities of compounds were evaluated by disc tests and diluted liquid methods. Results Nine cyclic-dipeptides and one dipeptide were identified as cyclo-[(S)-Pro-(R)-Leu] (T-1), seco-[(S)-Pro-(R)-Val] (T-2), cyclo-((S)-Leu--(S)-Val) (T-3), callyspondipeptide A (T-4), cyclo-[(S)-Pro-(R)-Phe] (T-5), cyclo-[(S)-Pro-(S)-Leu] (T-6), cyclo-[(S)-Pro-(S)-Val] (T-7), cyclo-[(S)-Pro-(S)-Ile] (T-8), cyclo-[(S)-Pro-(S)-Ala] (T-9) and cyclo-( Phe-Ser) (T-10). Antibacterial activity test showed that compounds T-5, T-7, and T-8 have an antibacterial effect on Staphylococcus aureus, with minimum inhibitory concentration (MIC) of 8, 4, and 8 μg/mL,respectively. Conclusion Compounds T-2, T-4, and T-10 were first isolated from this genus. Compounds T-5, T-7, and T-8 have significant antibacterial effects on Staphylococcus aureus. |
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