| 闫涵,杨佳依,许中秋,等.海洋来源硫酸多糖体外抗单纯疱疹病毒2型研究[J].中国海洋药物,2024,43(6):51-56. |
| 海洋来源硫酸多糖体外抗单纯疱疹病毒2型研究 |
| Study on the anti-Herpes simplex virus effect of marine polysaccharide SPM |
| 投稿时间：2023-04-23 修订日期：2023-06-02 |
| DOI：10.13400/j.cnki.cjmd.2024.06.007 |
| 中文关键词: 抗单纯疱疹病毒 海洋多糖SPM 灭活病毒 病毒增殖 |
| English Keywords:Anti-herpes simplex virus Marine polysaccharide SPM Inactivated virus Virus proliferation |
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| 中文摘要: |
| 目的 对海洋来源的化合物SPM进行体外抗HSV-2活性研究。方法 利用CPE抑制实验、Western blot实验和MTT法对其体外抗HSV-2活性和细胞毒性进行评价，此外，通过Western blot实验对其具体作用方式以及吸附后具体作用时间进行探究。最后，利用空斑减少、膜融合抑制和RT-PCR实验对其初步靶点进行探究。结果 SPM具有良好的体外抗HSV-2活性，其在Vero细胞中的IC50为16.1 ± 1.8 μg·mL-1，SI值大于62.1。此外，具体作用方式及吸附后具体作用阶段探究结果表明，SPM可以与病毒直接相互作用，还可以影响病毒的吸附、内吞以及增殖，发挥抗HSV-2作用。结论 SPM具有良好的体外抗HSV-2活性，具有高效低毒的特点，与现有的抗单纯疱疹病毒药物作用机制不同，为疱疹病毒感染的预防和治疗提供了新的研究思路和药物研发方向。 |
| English Summary: |
| Objective Anti-HSV-2 activity of marine compound SPM was studied in vitro. Methods CPE inhibition test, Western blot test and MTT assay were used to evaluate the anti-HSV-2 activity and cytotoxicity of SPM in vitro, respectively. In addition, the specific action mode and specific action time after adsorption were explored by Western blot test. Finally, plaque reduction, membrane fusion inhibition and RT-PCR experiments were used to explore its preliminary target. Results SPM has good anti-HSV-2 activity in vitro, the IC50 in Vero cells was 16.1 ± 1.8 μg·mL-1 and the SI value was greater than 62.1. In addition, the specific mode of action and the specific stage of action after adsorption show that SPM can directly interact with the virus to inactivate the virus, and can also affect the adsorption, endocytosis and proliferation of the virus to play an anti-HSV-2 role. Conclusion SPM has good anti-HSV-2 activity in vitro, with the characteristics of high efficiency and low toxicity, which is different from the existing anti-herpes simplex virus drugs, and provides new research ideas and drug research and development direction for the prevention and treatment of herpes virus infection. |
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