| 贺福杰,马骁尧,徐灿,等.ɩ-卡拉胶抗肠道病毒71型研究[J].中国海洋药物,2024,43(6):44-50. |
| ɩ-卡拉胶抗肠道病毒71型研究 |
| Study on the anti-enterovirus 71 activity of ?-Carrageenan |
| 投稿时间：2023-04-23 修订日期：2023-05-26 |
| DOI：10.13400/j.cnki.cjmd.2024.06.006 |
| 中文关键词: 抗肠道病毒71型 ?-卡拉胶 3C蛋白 VP1蛋白 |
| English Keywords:Anti-enterovirus 71 ?-Carrageenan 3C protein VP1 protein |
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| 中文摘要: |
| 目的 对海洋来源的化合物?-卡拉胶进行体外抗肠道病毒71型活性研究。方法 CPE抑制实验评价?-卡拉胶抗肠道病毒71型的活性，CPE实验探究?-卡拉胶的细胞毒性。通过空斑实验、Western blot实验、实时荧光定量PCR实验探究?-卡拉胶的抗病毒活性。利用Co-IP实验探究其作用靶点。结果 ?-卡拉胶具有高效低毒的抗肠道病毒71型活性，在Vero细胞中效果最好，IC50为6.29 μg·mL?1，CC50大于1 mg·mL?1，不同作用方式和不同作用时间实验表明，?-卡拉胶主要起作用于病毒吸附后阶段，且0~2 h给药可以显著抑制病毒VP1蛋白表达。此外，?-卡拉胶可以与病毒VP1蛋白结合，从而影响病毒感染细胞。结论 ?-卡拉胶具有良好的体外抗肠道病毒71型活性，为发现和改造新的海洋来源的糖类化合物提供了借鉴，为寻找新的抗肠道病毒71型药物提供了新思路。 |
| English Summary: |
| Objective In vitro studies on the anti-enterovirus 71 activity of the compound ?-carrageenan of marine origin. Methods CPE inhibition assay to evaluate the activity of ?-carrageenan against enterovirus 71, CPE assay to verify the mode of action and cytotoxicity of ?-carrageenan, combined with empty spot assay, Western blot assay and real-time fluorescence quantitative PCR assay for corroboration. Co-IP assay was used to verify its target of action. Results ?-Carrageenan has a highly efficient and low-toxic anti-enterovirus 71 activity and is most effective in Vero cells with an IC50 of 6.29 μg·mL-1 and a CC50 greater than 1 mg·mL-1. Experiments with different modes of action and different duration of action showed that ?-carrageenan acted mainly in the post-adsorption phase of the virus, and 0~2 h administration could significantly inhibit viral VP1 protein expression. In addition, ?-carrageenan could bind to the viral VP1 protein and thus affect virus-infected cells. Conclusion The good in vitro anti-enterovirus 71 activity of ?-carrageenan provides insights into the discovery and modification of new glycoconjugates of marine origin and offers new ideas for the search of new anti-enterovirus 71 drugs. |
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