| 岳亮光,黄会明,徐素玲,等.海洋链霉菌Streptomyces sp. S063中聚酮类化合物alnumycins的发现[J].中国海洋药物,2024,43(6):29-36. |
| 海洋链霉菌Streptomyces sp. S063中聚酮类化合物alnumycins的发现 |
| Discovery of alnumycins from the marine-derived Streptomyces sp. S063 |
| 投稿时间：2023-04-11 修订日期：2023-05-10 |
| DOI：10.13400/j.cnki.cjmd.2024.06.001 |
| 中文关键词: 海洋链霉菌 次级代谢产物 抗菌活性 聚酮 alnumycins |
| English Keywords:marine Streptomyces secondary metabolites anti-bacterial activity polyketide alnumycins |
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| 中文摘要: |
| 目的 挖掘海洋链霉菌中具有生物活性的次级代谢产物，为海洋药物的开发提供理论参考。方法 使用F2培养基对链霉菌进行大发酵，积累次级代谢产物。采用有机溶剂萃取、反相硅胶柱层析、半制备高效液相等分离手段对发酵产物进行分离纯化，通过质谱（MS）、核磁共振（NMR）数据分析以及文献比对，确定化合物结构，同时对所有化合物进行抗菌活性测试。运用生物信息学手段对Streptomyces sp. S063基因组中的生物合成基因簇进行预测和基因功能注释。结果 从海洋来源链霉菌Streptomyces sp. S063中分离得到4个聚酮类化合物alnumycin A（1）、alnumycin C（2）、alnumycin D（3）和K1115 A（4）。其中化合物1~3对多重耐药菌Staphylococcus aureus CCARM 3090展现出中等抑制活性。通过基因组生物信息学分析从Streptomyces sp. S063中定位到了alnumycin的生物合成基因簇（alm），并对1~4的生物合成途径进行了推测分析。结论 从海洋链霉菌Streptomyces sp. S063中挖掘到一类聚酮化合物alnumycins，具有潜在的药用价值，且其中所蕴含的其他化合物资源更有待深入挖掘。 |
| English Summary: |
| Objective Mining bioactive secondary metabolites from marine Streptomyces to provide theoretical reference for the development of marine drugs. Methods F2 medium was used for fermentation of Streptomyces to accumulate secondary metabolites. The crude extract was separated by ethyl acetate extraction，reverse phase silica gel chromatography and semi-preparative high performance liquid chromatography (HPLC). The structures of compounds were determined by MS analysis and NMR analysis as well as comparison with published literatures，and all compounds were tested for antibacterial activity. Bioinformatics methods were used to predict and annotate the biosynthetic gene clusters of Streptomyces sp. S063 genome. Results Three alnumycins compounds alnumycin A (1), alnumycin C (2) and alnumycin D (3), one anthraquinone compound K1115A (4) were isolated from marine Streptomyces sp. S063. Compounds 1?3 showed moderate inhibitory activity against Staphylococcus aureus CCARM 3090. The biosynthetic gene cluster of alnumycin (alm) was mapped from Streptomyces sp. S063 by bioinformatics analysis and the biosynthetic pathway of alnumycins in Streptomyces sp. S063 was proposed in combination with the literature. Conclusion A class of compounds with wide range of biological activities were excavated from marine Streptomyces sp. S063, which have potential medicinal value. At the same time, the biological value contained in marine Streptomyces sp. S063 needs to be further explored. |
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