| 高宇,邵盛培.海藻酸钠复合水凝胶的制备及其细菌检测应用研究[J].中国海洋药物,2022,41(6):49-56. |
| 海藻酸钠复合水凝胶的制备及其细菌检测应用研究 |
| Preparation of sodium alginate composite hydrogel for bacterial detection |
| 投稿时间：2022-09-27 修订日期：2022-10-25 |
| DOI：10.13400/j.cnki.cjmd.2022.06.001 |
| 中文关键词: 海藻酸钠 水凝胶 金纳米颗粒 细菌检测 |
| English Keywords:sodium alginate hydrogels gold nanoparticles bacterial detection |
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| 中文摘要: |
| 目的 制备海藻酸钠-金纳米颗粒复合水凝胶并研究其检测细菌的性能,为可视化细菌检测创伤敷料的开发奠定基础。方法 以羧海藻酸钠为原料,掺杂显色底物氯酚红β-D-吡喃半乳糖苷及半乳糖苷酶修饰的金纳米颗粒,通过钙离子引发海藻酸钠交联制备复合水凝胶,研究其对细菌的检测性能。结果 当金纳米颗粒尺寸为5纳米,浓度为100 nM时,水相中传感器对大肠杆菌和金黄色葡萄球菌的检测限可达100 细菌/mL；复合凝胶对金黄色葡萄球菌的检测限可达1000细菌/mL。结论 海藻酸钠-金纳米颗粒复合水凝胶具有生物相容性好细菌检测灵敏度高的优点,有望作为伤口敷料用于检测伤口愈合过程中的细菌感染。 |
| English Summary: |
| Objective Sodium alginate-gold nanoparticle composite hydrogels were prepared and their performance in detecting bacteria were studied, which lay a foundation for the development of visualized bacterial detection for wound dressings. Methods Sodium carboxyalginate were doped with chromogenic substrate chlorophenol red β-D-galactopyranoside and galactosidase-modified gold nanoparticles, and were crosslinked to form the hydrogel under the induction of Ca2+. Subsequently, the capability of bacterial detection was studied. Results The limit of detection for Escherichia coli and Staphylococcus aureus in the aqueous phase was determined as 100 bacteria/mL when the concentration of gold nanoparticles was 100 nM. The detection limit of composite gel for Staphylococcus aureus was as low as 1000 bacteria/mL. Conclusion Sodium alginate-gold nanoparticle composite hydrogel has the advantage of good biocompatibility and high sensitivity for bacterial detection, which holds great promise as a wound dressing for detecting bacterial infections during wound healing. |
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