| 岳贤琳,杨郁,赵雪,等.产黄青霉Penicillium chrysogenum S-3-25人工海水培养基发酵次级代谢产物研究[J].中国海洋药物,2022,41(6):1-9. |
| 产黄青霉Penicillium chrysogenum S-3-25人工海水培养基发酵次级代谢产物研究 |
| The secondary metabolites isolated from Penicillium chrysogenum S-3-25 fermented in artificial seawater medium |
| 投稿时间：2022-06-30 修订日期：2022-08-21 |
| DOI：10.13400/j.cnki.cjmd.2022.06.010 |
| 中文关键词: 海洋来源真菌 产黄青霉 人工海水培养基 代谢产物 细胞毒 |
| English Keywords:marine-derived fungus Penicillium chrysogenum artificial seawater medium metabolites cytotoxic activity |
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| 中文摘要: |
| 目的 阐明南极深海来源真菌产黄青霉Penicillium chrysogenum S-3-25人工海水培养基的发酵次级代谢产物及其活性。方法 利用各种色谱技术分离纯化次级代谢产物，根据理化和波谱数据（核磁共振、质谱技术和Marfey分析）鉴定化合物结构，采用3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide（MTT）法评价细胞毒活性。结果 从真菌S-3-25人工海水培养基发酵产物中分离鉴定了8个单体化合物：2-[[(2S)-2-amino-1-oxopropyl] amino] benzoic acid (1)，methyl 2-[[(2S)-2-amino-1-oxopropyl] amino] benzoate (2)，2-[[(2S)-2-hydroxy-1-oxopropyl] amino] benzamide (3)，3-(p-hydroxyphenyl)-N-methylpropionamide (4)，4-hydroxycinnamamide (5)，cyclo-(L-Pro-L-Tyr) (6)，脑苷脂A (7) 及脑苷脂B (8)。细胞毒活性测试结果表明化合物1~8在50 μM作用浓度下对三种受试细胞（人乳腺癌MCF-7，人肺癌A549以及小鼠小胶质BV2细胞）的抑制率均低于50%。结论 从极地深海来源真菌产黄青霉S-3-25人工海水培养基发酵产物中分离得到8个单体化合物，其中化合物1和2为新天然产物，未见有文献数据的报道。化合物1~8均未呈现较强的细胞毒活性。 |
| English Summary: |
| Objective To investigate the secondary metabolites of the fungus Penicillium chrysogenum S-3-25 isolated from polar deep sea and fermented in artificial seawater medium. Methods The secondary metabolites were isolated by multiple separation techniques, the structures of the isolated compounds were identified according to the physicochemical and spectral data (nuclear magnetic resonance, mass spectrometry and Marfey’s analyses), the cytotoxic activities were assayed by MTT method. Results Eight metabolites, 2-[[(2S)-2-amino-1-oxopropyl] amino] benzoic acid (1), methyl 2-[[(2S)-2-amino-1-oxopropyl] amino] benzoate (2), 2-[[(2S)-2-hydroxy-1-oxopropyl] amino] benzamide (3), 3-(p-hydroxyphenyl)-N-methylpropionamide (4), 4-hydroxycinnamamide (5), cyclo-(L-Pro-L-Tyr) (6), cerebroside A (7) and cerebroside B (8), were isolated and identified from the artificial seawater fermentation products. Compounds 1–8 showed weak cytotoxicities against the tested MCF-7, A549 and BV2 cells. Conclusion Eight compounds were further isolated from the fungus Penicillium chrysogenum S-3-25. Compounds 1 and 2 were new natural products and their NMR data were reported for the first time. Compounds 1–8 did not show strong cytotoxic activity. |
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