| 胡洁,武艳伟,王翠芳,等.1株红树来源真菌Penicillium sp.次级代谢产物及其生物活性研究（英文）[J].中国海洋药物,2023,42(4):13-18. |
| 1株红树来源真菌Penicillium sp.次级代谢产物及其生物活性研究（英文） |
| Secondary metabolites of the mangrove-derived fungus Penicillium sp. and their biological activities |
| 投稿时间：2022-05-04 修订日期：2022-05-26 |
| DOI：10.13400/j.cnki.cjmd.2023.04.011 |
| 中文关键词: 青霉属 次级代谢产物 斑马鱼血管抑制活性 |
| English Keywords:Penicillium sp. secondary metabolite zebrafish anti-angiogenic activity |
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| 中文摘要: |
| 目的 研究1株红树来源真菌Penicillium sp.中的次级代谢产物及生物活性。方法 综合运用硅胶柱层析和半制备型HPLC等方法分离纯化次级代谢产物，利用NMR及MS等现代波谱分析方法鉴定化合物结构，以斑马鱼血管抑制模型对其进行活性评价。结果 在细胞毒活性的指导下，从Penicillium sp.中分离出抗肿瘤活性化合物brefeldin A，同时还分离出5个天然产物，分别是 (+)-semivioxanthin (1)、(-)-striatisporolide A (2)、dibutyl phthalate (3)、bisdethiobis (methylthio)-acetylapoaranotin (4) 和alternarosin A (5)。其中化合物1和3分别在1 μmol/L和10 μmol/L浓度下显示出斑马鱼血管抑制活性，但同时会导致一定的脊柱弯曲。化合物3还具有抑制硅藻附着的潜在防污活性。结论 化合物4和5为首次从真菌Penicillium sp.中分离获得。化合物1和3具有潜在的生物活性。 |
| English Summary: |
| Objective To study the secondary metabolites from the mangrove-derived fungus Penicillium sp. and evaluate their biological activity. Methods The compounds were separated and purified by silica gel column chromatography (CC) and semi-preparative HPLC. Their structures were identified by modern spectroscopic analysis including NMR and MS methods. All the compounds were tested for zebrafish anti-angiogenic and anti-microalgae activities. Results Under the guidance of cytotoxic activity, brefeldin A, an anti-tumor activity natural compound, and five compounds including (+)-semivioxanthin (1), (-)-striatisporolide A (2), dibutyl phthalate (3), bisdethiobis (methylthio)-acetylapoaranotin (4) and alternarosin A (5) were obtained from the fungus Penicillium sp. Compounds 1 and 3 exhibited anti-angiogenic activity to zebrafish embryo at the concentration of 1 and 10 μmol/L, associated with notochord malformation and tail malformation (28 h). Compound 3 showed potential antifouling activity against Navicula leavissima. Conclusion Compounds 4 and 5 were isolated from fungus Penicillium sp. for the first time and compounds 1 and 3 showed potential biological activity. |
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