韩青,周涵,王维雯,等.两种颜色仿刺参Apostichopus japonicus中糖胺聚糖表达谱的差异性研究[J].中国海洋药物,2023,42(2):1-9.
两种颜色仿刺参Apostichopus japonicus中糖胺聚糖表达谱的差异性研究
Differences of glycosaminoglycans expression profiles of Apostichopus japonicus in different colors
投稿时间:2022-03-15  修订日期:2022-04-10
DOI:
中文关键词:  液质联用技术  糖胺聚糖  仿刺参  差异性比较
English Keywords:LC-MS/MS  Glycosaminoglycans  Apostichopus japonicus  difference comparison
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作者单位E-mail
韩青 中国海洋大学 731216750@qq.com 
周涵 中国海洋大学 judy199807@163.com 
王维雯 中国海洋大学 wangweiwen97@163.com 
黄曦玥 中国海洋大学 huangxiyue@simm.ac.cn 
陈刚 青岛金海富源海洋实业有限公司 gangchen202203@163.com 
陈四清 中国水产科学研究院黄海水产研究所 chensq@ysfri.ac.cn 
于广利 中国海洋大学 glyu@ouc.edu.cn 
李国云* 中国海洋大学 liguoyun@ouc.edu.cn 
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中文摘要:
      目的 基于液质联用和糖组学分析技术,分析比较不同颜色仿刺参中的糖胺聚糖,获得不同颜色仿刺参的糖胺聚糖表达谱信息,从而完善海参糖链表达谱信息,促进无脊椎动物的糖链研究。方法 首先对两种颜色仿刺参进行脱脂和蛋白酶酶解处理,然后采用离子交换柱纯化以去除蛋白和高硫酸化聚糖等杂质并获得糖胺聚糖。采用GAGs酶解获得GAGs二糖并对其进行2-AMAC荧光标记,再基于RP-C18-MS/MS-MRM方法进行GAGs表达谱差异分析。结果 在两种颜色仿刺参中CS含量均最高,为2.5~4.5 μg/g,HA含量次之,HS的含量最低。海参中GAGs总量远低于海参中岩藻糖基化硫酸软骨素和岩藻聚糖硫酸酯的含量,白色仿刺参的GAGs总量明显高于青色仿刺参。两种仿刺参中CS二糖以CS-0S、CS-4S、CS-6S为主,HS二糖主要是HS-NS6S和HS-TriS,但是两者在GAGs二糖含量、百分含量、硫酸化模式和硫酸化程度上均存在较大差异。结论 本文首次获得不同颜色仿刺参糖胺聚糖表达谱信息,有助于了解不同颜色仿刺参来源内源性糖链信息,为海参活性成分的研究提供依据。
English Summary:
      Objective Based on liquid chromatography-mass spectrometry and glycomic analysis techniques, the expression profiles of glycosaminoglycans of Apostichopus japonicus in different colors were analyzed and compared. Glycosaminoglycans expression information of Apostichopus japonicus in different colors was obtained so as to improve the glycans expression information of sea cucumber and promote the glycans research of invertebrates. Methods First, the two colors Apostichopus japonicus were degreased and treated by Pronase, and then purified by ion exchange column to remove protein and sulfated polysaccharides and obtain glycosaminoglycans. GAGs disaccharides were obtained by GAGs enzymesSand labeled with 2-AMAC fluorescence, and then the difference of GAGs expression profiles were analyzed based on RP-C18-MS/MS-MRM method. Results CS was the highest content in the range of 2.5~4.5 μg/g, followed by HA, HS was the lowest content in two colors Apostichopus japonicus. The total amount of GAGs in sea cucumber was much lower than the contents of fucosylated chondroitin sulfate and Fucoidan in sea cucumber, the total amount of GAGs in white Apostichopus japonicus was significantly higher than that in green Apostichopus japonicus. CS-0S, CS-4S and CS-6S were the main CS disaccharides in the two kinds of Apostichopus japonicus, while HS-NS6S and HS-TriS were the main HS disaccharides. However, there were significant differences between two colors Apostichopus japonicus in disaccharides content, percentage content, sulfated modification mode and the degree of sulfated modification of GAGs. Conclusion In this paper, the glycosaminoglycans expression profiles of Apostichopus japonicus in different colors were obtained for the first time, which was helpful to understand the endogenous glycans information of Apostichopus japonicus in different colors and provide basis for the study of active ingredients of sea cucumber.
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