| 吕昌宁,孙延龙,张敏,等.岩藻多糖体外抗KRV活性初步研究（英文）[J].中国海洋药物,2022,41(3):69-74. |
| 岩藻多糖体外抗KRV活性初步研究（英文） |
| Preliminary Study on Anti-KRV Activity of Fucoidan in Vitro |
| 投稿时间：2022-03-09 修订日期：2022-05-04 |
| DOI：10.13400/j.cnki.cjmd.2022.03.013 |
| 中文关键词: 岩藻多糖 KRV IL-1 IL-6 |
| English Keywords:Fucoidan KRV IL-1 IL-6 |
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| 中文摘要: |
| 目的 为了研究岩藻多糖体外抗Kilham rat virus（KRV）病毒的活性以及抑制炎症因子IL-1、IL-6的表达。方法 CCK-8法测得岩藻多糖对大鼠肾细胞（NRK）的半数细胞毒性浓度（CC50）；通过细胞病变效应测定药物的抗病毒活性，计算药物的半数抑制浓度（IC50）以及治疗指数（SI）；建立KRV感染大鼠肾细胞（NRK）细胞模型，设置阴性对照、病毒感染组以及治疗实验组，通过实时荧光定量PCR检测病毒以及IL-1、IL-6的表达水平。结果 CCK-8法测得药物的CC50 > 2500μg/mL，岩藻多糖的IC50为114.8±8.1μg/mL，治疗指数 > 21.78；qPCR结果表明KRV的转录水平实验组较阳性对照组明显降低，且IL-1、IL-6的转录水平亦显著降低。结论 岩藻多糖具有较小的细胞毒性以及良好的抗KRV病毒的效果，并且能够减轻病毒诱导的炎症反应，为防治病毒感染导致的糖尿病提供理论基础。 |
| English Summary: |
| Objective To study the anti-Kilham Rat virus (KRV) activity and the inhibition effect with the expression of inflammatory factors IL-1 and IL-6 of fucoidan in vitro. Methods The 50% cytotoxic concentrations (CC50) of fucoidan on rat kidney cells (NRK) were measured by CCK-8 method. The antiviral activity of the drug was determined by cytopathic effect. The half inhibitory concentration (IC50) and selectivity index (SI) of the drug were calculated. The expression levels of virus, IL-1 and IL-6 were detected by real-time quantitative PCR (qPCR). Results The CC50 of CCK-8 was over 2500μg/mL, the IC50 and SI of fucoidan were 114.8±8.1 μg/mL and over 21.78, respectively. The qPCR assay showed that the transcription level of KRV in the experimental group was significantly lower than that in the positive control group, and the transcription levels of IL-1 and IL-6 were also significantly lower. Conclusion Fucoidan has less cytotoxicity and good anti-KRV effect, and it can reduce the inflammatory reaction induced by the virus, which provides a theoretical basis for the prevention and treatment of diabetes caused by viral infection. |
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