朱俊衡,宋蒙蒙,胡怡伟,等.深海橘青霉Penicillium citrinum SCSIO41305化学成分及其乙酰胆碱酯酶抑制活性研究[J].中国海洋药物,2022,41(5):27-31.
深海橘青霉Penicillium citrinum SCSIO41305化学成分及其乙酰胆碱酯酶抑制活性研究
Chemical constituents of a deep-sea derived Penicillium citrinum SCSIO41305 and their AchE inhibitory activities evaluation
投稿时间：2021-08-19  修订日期：2021-10-07
DOI：
中文关键词:  深海橘青霉Penicillium citrinum，乙酰胆碱酯酶，次级代谢产物
English Keywords:deep-sea  Penicillium citrinum, acetylcholinesterase, secondary  metabolites
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作者单位E-mail
朱俊衡 西北大学 化工学院 895770790@qq.com 
宋蒙蒙 ：西北大学 化工学院 1196638353@qq.com 
胡怡伟 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室 872401815@qq.com 
张晓勇 华南农业大学 海洋学院 zhangxiaoyong@scau.edu.cn 
刘庆超 ：西北大学 化工学院 liuqc21@nwu.edu.cn 
王俊锋* 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点实验室 wangjunfeng@scsio.ac.cn 
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中文摘要:
      研究1株采自深海沉积物中橘青霉Penicillium citrinum SCSIO41305的次级代谢产物。方法 利用薄层色谱法（TLC）、硅胶柱层析法、凝胶柱层析法、中压制备液相色谱法（MPLC）、高效液相色谱法（HPLC）等分离方法对该菌株发酵产物进行了分离纯化；通过核磁共振（NMR）等现代波谱学方法，同时结合相关文献进行数据分析，鉴定化合物结构。结果 从橘青霉Penicillium citrinum（SCSIO41305）的代谢产物中分离得到7个化合物，分别是citrininc（1）、ergosterol（2）、penicillenol A1（3）、penicillenol A2（4）、penicitrinone A（5）、ozazino-cyclo-(2,3-dihydroxyl-trp-tyr)（6）、3,4,5-trimethylbenzene-1,2-diol（7）。活性测试结果表明，化合物2对金黄色葡萄球菌有一定的抑制作用，MIC值为0.50 mg/mL，化合物3和4对乙酰胆碱酯酶有较好的抑制作用，其IC50分别为10.43和15.41 μmol/L。
English Summary:
      Objective To study the secondary metabolites of the Penicillium citrinum SCSI41305 collected from the deep sea sediments. Methods The secondary metabolites of the strain were separated and purified by thin layer chromatography (TLC), silica gel column chromatography, gel column chromatography, medium pressure liquid chromatography (MPLC). Through modern spectroscopy methods such as NMR and LCMS, combined with relevant literature data analysis the structure of the compound were identified. Results Seven compounds were isolated from the secondary metabolites of Penicillium citrinum SCSIO41305, which were identified as citrinin (1), ergosterol (2), penicillinol A1 (3), penicillinol A2 (4), penicitrinone A (5), cyclo-(L-tryptophyl-L-phenylalanyl) (6) and 3,4,5-trimethylbenzene-1,2-diol (7). Bioactivity tests showed that compound 2 exhibited antibacterial against Staphylococcus aureus with the MIC value of 0.50 mg/mL. In addition, compounds 3 and 4 exhibited inhibition against acetylcholinesterase with the IC50 values of 10.43 and 15.41 μM, respectively, which was reported for the first time.
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