| 李旺盛,高天润,章海燕,等.西瑁岛软珊瑚Dendronephthya sp.的化学成分和生物活性研究[J].中国海洋药物,2022,41(2):7-13. |
| 西瑁岛软珊瑚Dendronephthya sp.的化学成分和生物活性研究 |
| Chemical constituents and bioactivities of soft coral Dendronephthya sp. from Ximao Island |
| 投稿时间：2021-04-23 修订日期：2021-06-02 |
| DOI： |
| 中文关键词: 软珊瑚 甾体 细胞毒性 抗炎 |
| English Keywords:soft coral steroids cytotoxic anti-inflammatory. |
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| 中文摘要: |
| 目的 对采自中国南海西瑁岛海域的软珊瑚Dendronephthya sp.的化学成分和抗炎活性研究。方法 综合利用薄层色谱、硅胶柱色谱和凝胶柱层析等分离手段,对纯化的单体成分采用核磁波谱(NMR)和质谱(MS)等方法进行鉴定,结合文献数据比对确定化合物结构；并对化合物进行了抗炎活性测试和细胞毒性实验。结果 从软珊瑚Dendronephthya sp.中共分离鉴定了11个化合物,包括6个甾体化合物,4个嘧啶类化合物及1个醇类化合物：胆固醇(1),胆固醇乙酸脂(2),胆固醇-5α,6α-环氧化物(3),鲨肝醇(4),11-acetoxy-3β,6α-dihydroxy-9,11-seco-5α-cholest-7-en-9-one(5),啤酒甾醇(6),3β,5α,6β-三羟基胆甾烷醇(7),胸腺嘧啶(8),脲嘧啶(9),胸苷(10),脲苷(11)。结论 化合物5和6是首次从该种属中分离得到,其中化合物5对脂多糖诱导的BV-2细胞的炎症反应具有抑制作用。 |
| English Summary: |
| Objective To study the chemical constituents and bioactivities of soft coral Dendronephthya sp. collected from Ximao Island in South China Sea. Methods The isolation and purification of these compounds were performed by TLC, silica gel and Sephadex LH-20 column chromatography. Their structures were characterized by analysis of NMR and MS data, as well as by comparison with the data reported in literature and the anti-inflammatory activity of the pure compounds was tested. Results Eleven compounds were isolated from the soft coral Dendronephthya sp. and their structures were identified as cholesterol (1),cholesterol acetate (2), cholesterol-5α,6α-Epoxide (3), squaliol (4), 11-acetoxy-3β,6α-dihydroxy-9,11-seco-5α-cholest-7-en-9-one (5), beer sterol (6), 3β,5α,6β-trihydroxycholestanol (7), thymine (8), uracil (9), thymidine (10), uridine (11). Conclusion Compounds 5 and 6 were first isolated from the genus coral, of which compound 5 could inhibit lipopolysaccharide induced inflammation in BV-2 microglial cells. |
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