| 邢丽,常一民,韩亚欣,等.南海来源放线菌Streptomyces sp. HDN1844000中lobophorin N的分离与结构鉴定[J].中国海洋药物,2021,40(5):9-15. |
| 南海来源放线菌Streptomyces sp. HDN1844000中lobophorin N的分离与结构鉴定 |
| Isolation and structure elucidation of lobophorin N from the South China Sea-derived Streptomyces sp. HDN1844000 |
| 投稿时间：2021-02-03 修订日期：2021-03-30 |
| DOI： |
| 中文关键词: 抑菌 lobphorins 海洋链霉菌 分子网络 中国南海 |
| English Keywords:antibacterial lobphorins marine Streptomyces molecular networking South China Sea |
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| 中文摘要: |
| 摘 要：目的 对南海来源放线菌Streptomyces sp. HDN1844000的次级代谢产物进行研究。方法 在生物活性筛选和液相二级质谱（LC-MS/MS）分子网络化学信息学策略指导下，采用硅胶柱层析、Sephadex LH-20凝胶层析和高效液相色谱法（HPLC），对菌株提取物进行分离纯化，通过质谱（MS）、核磁共振谱（NMR）分析和与文献比对，阐明化合物结构，并对化合物的抑菌活性进行评价。结果 从放线菌Streptomyces sp. HDN1844000的次级代谢产物中分离出1个新的spirotetronate类抗生素lobophorin N（1）和1个已知的类似物lobophorin A（2）。化合物1是1个新的lobophorin衍生物，其带有1个含3-氨基的D-kijanose变体并且在C-32处有1个甲基。生物活性筛选结果表明化合物1对蜡状芽孢杆菌、变形杆菌、草分枝杆菌和副溶血弧菌具有良好的抑菌活性，最低抑菌浓度（MIC）值为0.10～0.39 μmol/L。 |
| English Summary: |
| Abstract: Objective To study the metabolites of South China Sea-derived Streptomyces sp. HDN1844000. Methods The strain extracts were isolated and purified by repeated column chromatography across silica gel, Sephadex LH-20, and HPLC, under the guidance of bioassay screening and cheminformatics strategy of LC-MS/MS molecular networking. The structures of compounds were elucidated by extensive MS, NMR spectra analyses and comparison with literatures. The antibacterial activities of all compounds were evaluated. Results One new spirotetronate antibiotic lobophorin N (1) and one known analogue lobophorin A (2) were isolated from the actinomycete Streptomyces sp. HDN1844000. Compound 1 is a new lobophorin derivative which carries D-kijanose variant with a 3-amino group and a methyl group at C-32. Bioactivity screening of 1 indicated promising antibacterial activities against bacillus cereus, proteus species, mycobacterium phlei, and vibrio parahaemolyticus, with MIC values ranging from 0.10 to 0.39 μmol/L. |
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