文章摘要
刘碧丽,丘鹰昆,颜志文,等.海洋来源青霉菌Penicillium polonicum H92次级代谢产物的研究[J].中国海洋药物,2021,40(4):27-32.
海洋来源青霉菌Penicillium polonicum H92次级代谢产物的研究
Studies on marine secondary metabolites of fungus Penicillium polonicum H92
投稿时间:2021-01-04  修订日期:2021-02-27
DOI:
中文关键词: 青霉菌Penicillium polonicum  次生代谢产物  结构鉴定  抗流感病毒
英文关键词: Penicillium polonicum  secondary metabolites  structure identification  anti-influenza virus
基金项目:
刘碧丽  丘鹰昆  颜志文  吴小枫  邱彦  杨嘉永
厦门大学 附属厦门市第一医院药学部,厦门大学 药学院,厦门大学 附属厦门市第一医院药学部,厦门大学 附属厦门市第一医院药学部,厦门大学 医学院,厦门大学 附属厦门市第一医院药学部
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中文摘要:
      摘要:目的 对源自福建漳江口红树林底部海水沉积物的青霉菌Penicillium polonicum H92的次级代谢产物进行化学成分和生物活性研究。方法 综合利用有机溶剂萃取、硅胶柱色谱、反相硅胶(ODS)柱色谱、凝胶(Sephadex LH-20)柱色谱、薄层色谱、半制备高效液相色谱等多种手段对青霉菌Penicillium polonicum H92的次生代谢产物进行分离纯化;采用质谱(MS)、核磁共振谱(NMR)、紫外光谱(UV)等现代波谱技术鉴定所分离的化学成分结构;以奥司他韦为阳性对照药物,对上述化合物进行抗流感病毒神经氨酸酶(NA)活性测试。结果 从青霉菌Penicillium polonicum H92中分离得到7个次生代谢产物,其中4个喹啉酮类化合物:纯绿青霉素(1),3-甲氧基纯绿青霉素(2),纯绿青霉素醇(3),喹诺酮(4);2个二酮哌嗪类化合物:Fructigenine A(5),环(色-苯丙)二肽(6);一个生物碱:橙黄胡椒酰胺C(7)。结论 生物活性初步评价结果显示,化合物2和6对流感病毒神经氨酸酶具有不同程度的抑制活性。
英文摘要:
      Abstract: Objective To study the chemical composition and biological activity of secondary metabolites of Penicillium polonicum H92 from seawater sediments at the bottom of mangroves in Zhangjiang Estuary, Fujian. Methods Silica gel column chromatography, reversed-phase silica gel (ODS) column chromatography, gel (Sephadex LH-20) column chromatography, thin-layer chromatography, and semi-preparative high-performance liquid chromatography were used to analyze the organic solvent extraction of Penicillium polonicum H92, and the secondary metabolites were isolated and purified. Mass spectrometry (MS), nuclear magnetic resonance (NMR), ultraviolet spectroscopy (UV) and other modern spectroscopy techniques were used to identify the structure of the separated chemical constituents. Oseltamivir was used as a positive control drug to conduct neuraminidase (NA) activity test against influenza virus with the above compounds. Results Seven secondary metabolites were isolated from Penicillium polonicum H92, and four of which were identified as quinolinone compounds as Viridicatin (1), 3-O-Methylviridicatin (2), and Viridicatol (3), 3-Hydroxy-4-(3-methoxyphenyl)-2-quinolinone (4). Compound 5 and 6 were diketopiperazines as Cyclic (L-Phe-L-Trp) (5), and Fructigenine A (5). Compoundone 7 was elucidated as an alkaloid of Aurantiomide C. Conclusion The preliminary evaluation of biological activity showed that compounds 2 and 6 had different levels of inhibitory activity against influenza virus neuraminidase.
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