| 杨胜涛,刘怡,萧振邦,等.褐藻苷苔多酚7-Polyphenol-Ecklonia抑制肿瘤转移和血管新生的研究[J].中国海洋药物,2021,40(2):35-42. |
| 褐藻苷苔多酚7-Polyphenol-Ecklonia抑制肿瘤转移和血管新生的研究 |
| Study on the inhibitory effect of 7-Polyphenol-Ecklonia of the brown algae Ecklonia cava on tumor metastasis and angiogenesis |
| 投稿时间：2020-06-28 修订日期：2020-07-28 |
| DOI： |
| 中文关键词: 褐藻多酚 肿瘤转移 血管新生 金属基质蛋白酶 低氧诱导因子-1α |
| English Keywords:Brown algae polyphenols Tumor metastasis Angiogenesis MMPs HIF-1α |
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| 中文摘要: |
| 目的 探究海洋褐藻苷苔(Ecklonia cava)多酚7-polyphenol-ecklonia(7PE)对肿瘤侵袭转移和血管生成的影响。方法 采用细胞毒性实验检测7PE分别对人成纤维肉瘤细胞(HT-1080)和人脐静脉内皮细胞(HUVEC)的毒性作用；划痕实验检测7PE对HT-1080细胞的迁移能力影响；明胶酶谱法和ELISA检测HT-1080细胞的金属基质蛋白酶-2/9(MMP-2/9)、低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)蛋白的表达水平；成管实验检测7PE对HUVEC成管的作用；分子对接模拟7PE与MMP-2/9和HIF-1α蛋白的相互作用。结果 7PE明显抑制HT-1080的迁移和MMP-2、MMP-9、VEGF和HIF-1α蛋白的表达；且能明显抑制HUVEC成管能力；此外与MMP-2/9和HIF-1α能形成稳定的相互作用。结论 7PE可抑制肿瘤细胞转移和血管新生,可作为研发抗肿瘤药物的活性物质。 |
| English Summary: |
| Objective To explore the effect of marine brown algae Ecklonia cava polyphenol 7-polyphenol-ecklonia (7PE) on tumor invasion and metastasis and angiogenesis. Methods Cytotoxicity experiments were used to detect the toxic effects of 7PE on human fibrosarcoma cells (HT-1080) and human umbilical vein endothelial cells (HUVEC). The scratch test was used to examine the effect of 7PE on the migration ability of HT-1080 cells. Gelatin zymography and ELISA were used to detect metal matrix proteinase-2/9 (MMP-2/9), hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein expression level in HT-1080 cells. Tube forming experiment was used to detect the effect of 7PE on HUVEC tube forming. Molecular docking was used to simulate the interaction of 7PE with MMP-2/9 and HIF-1α protein, respectively. Results 7PE obviously inhibited the migration of HT-1080 and the expression of MMP-2/9, VEGF, HIF-1α protein. And 7PE obviously inhibited the HUVEC tube forming ability. Moreover, it can form stable interaction with MMP-2/9 and HIF-1α. Conclusion 7PE can inhibit tumor cell metastasis and angiogenesis, and can be used as an active substance in the development of anti-tumor drugs. |
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