文章摘要
徐璐瑶,董玉静,鲍依蕾,等.深海链霉菌Streptomyces sp. OUCT16-23中放线菌素类次级代谢产物的研究[J].中国海洋药物,2021,40(4):40-44.
深海链霉菌Streptomyces sp. OUCT16-23中放线菌素类次级代谢产物的研究
Study on the actinomycin-type secondary metabolites from Streptomyces sp. OUCT16-23
投稿时间:2020-06-24  修订日期:2020-08-02
DOI:
中文关键词: 深海链霉菌  多重耐药  放线菌素
英文关键词: marine Streptomyces, multi-drug resistance, actinomycin
基金项目:国家重点研发项目(2019YFC0312501);国家自然科学基金-山东省联合海洋科学研究(U1706206);青岛市民生科技计划项目(17-3-3-44-nsh)资助
徐璐瑶  董玉静  鲍依蕾  金恩敬  李文利  李花月
中国海洋大学,中国海洋大学 教育部海洋药物重点实验室,中国海洋大学 教育部海洋药物重点实验室,中国海洋大学 教育部海洋药物重点实验室,中国海洋大学 教育部海洋药物重点实验室,中国海洋大学 教育部海洋药物重点实验室
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中文摘要:
      目的 对一株深海链霉菌Streptomyces sp. OUCT16-23的次级代谢产物进行研究,发现具有新颖化学结构和良好生物活性的化合物。方法 采用V6培养基对Streptomyces sp. OUCT16-23进行发酵培养,经高效液相色谱(HPLC)纯化得到化合物1和2,并综合运用质谱(HR-ESI-MS)、核磁共振(NMR)等波谱学方法进行结构鉴定。结果 从深海链霉菌Streptomyces sp. OUCT16-23中分离得到2个放线菌素类化合物,actinomycin D(1)和actinomycin V(2),并发现其对多重耐药菌Staphylococcus aureus CCARM 3090,Enterococcus faecalis CCARM 5172,Enterococcus faecium CCARM 5203和Klebsiella pneumoniae ATCC 13883具有显著的抑制活性。结论 从Streptomyces sp. OUCT16-23中分离鉴定了具有优良抗多重耐药菌活性的 2个放线菌素类化合物,为筛选放线菌素类抗生素高产菌株奠定了基础。
英文摘要:
      Objective To obtain secondary metabolites with novel structures and significant bioactivity from Streptomyces sp. OUCT16-23. Methods Streptomyces sp. OUCT16-23 was cultured with V6 medium, and compounds 1 and 2 were obtained with HPLC purification. The structures of compounds 1 and 2 were identified using the spectroscopic methods including MS and NMR. Results Two actinomycin-type compounds were obtained from Streptomyces sp. OUCT16-23, which were identified as actinomycin D (1) and actinomycin V (2), respectively. Compounds 1 and 2 exhibited significant inhibitory activities against multi-drug resistant bacteria (MDRB): Staphylococcus aureus CCARM 3090, Enterococcus faecalis CCARM 5172, Enterococcus faecium CCARM 5203 and Klebsiella pneumoniae ATCC 13883. Conclusion Two actinomycin-type compounds with significant anti-MDRB activities were isolated and identified from Streptomyces sp. OUCT16-23, which laid a foundation for screening high-yield actinomycin producing strains.
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