| 徐璐瑶,董玉静,鲍依蕾,等.深海链霉菌Streptomyces sp. OUCT16-23中放线菌素类次级代谢产物的研究[J].中国海洋药物,2021,40(4):40-44. |
| 深海链霉菌Streptomyces sp. OUCT16-23中放线菌素类次级代谢产物的研究 |
| Study on the actinomycin-type secondary metabolites from Streptomyces sp. OUCT16-23 |
| 投稿时间：2020-06-24 修订日期：2020-08-02 |
| DOI： |
| 中文关键词: 深海链霉菌 多重耐药 放线菌素 |
| English Keywords:marine Streptomyces, multi-drug resistance, actinomycin |
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| 中文摘要: |
| 目的 对一株深海链霉菌Streptomyces sp. OUCT16-23的次级代谢产物进行研究，发现具有新颖化学结构和良好生物活性的化合物。方法 采用V6培养基对Streptomyces sp. OUCT16-23进行发酵培养，经高效液相色谱（HPLC）纯化得到化合物1和2，并综合运用质谱（HR-ESI-MS）、核磁共振（NMR）等波谱学方法进行结构鉴定。结果 从深海链霉菌Streptomyces sp. OUCT16-23中分离得到2个放线菌素类化合物，actinomycin D（1）和actinomycin V（2），并发现其对多重耐药菌Staphylococcus aureus CCARM 3090，Enterococcus faecalis CCARM 5172，Enterococcus faecium CCARM 5203和Klebsiella pneumoniae ATCC 13883具有显著的抑制活性。结论 从Streptomyces sp. OUCT16-23中分离鉴定了具有优良抗多重耐药菌活性的 2个放线菌素类化合物，为筛选放线菌素类抗生素高产菌株奠定了基础。 |
| English Summary: |
| Objective To obtain secondary metabolites with novel structures and significant bioactivity from Streptomyces sp. OUCT16-23. Methods Streptomyces sp. OUCT16-23 was cultured with V6 medium, and compounds 1 and 2 were obtained with HPLC purification. The structures of compounds 1 and 2 were identified using the spectroscopic methods including MS and NMR. Results Two actinomycin-type compounds were obtained from Streptomyces sp. OUCT16-23, which were identified as actinomycin D (1) and actinomycin V (2), respectively. Compounds 1 and 2 exhibited significant inhibitory activities against multi-drug resistant bacteria (MDRB): Staphylococcus aureus CCARM 3090, Enterococcus faecalis CCARM 5172, Enterococcus faecium CCARM 5203 and Klebsiella pneumoniae ATCC 13883. Conclusion Two actinomycin-type compounds with significant anti-MDRB activities were isolated and identified from Streptomyces sp. OUCT16-23, which laid a foundation for screening high-yield actinomycin producing strains. |
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