程玉静,唐旭利,李平林,等.1株深海来源Aspergillus versicolor (MCCC 3A00937)化学成分及生物活性研究[J].中国海洋药物,2021,40(1):23-28.
1株深海来源Aspergillus versicolor (MCCC 3A00937)化学成分及生物活性研究
Study on the chemical composition and biological activity of a strain of Aspergillus versicolor (MCCC 3A00937) from deep sea
投稿时间：2020-03-27  修订日期：2020-05-06
DOI：
中文关键词:  深海真菌  Aspergillus versicolor  次级代谢产物
English Keywords:deep sea fungus  Aspergillus versicolor  secondary metabolites
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作者单位E-mail
程玉静 中国海洋大学 海洋药物教育部重点实验室 1056199942@qq.com 
唐旭利 中国海洋大学 化学化工学院  
李平林 中国海洋大学 海洋药物教育部重点实验室 医药学院  
李国强* 中国海洋大学 海洋药物教育部重点实验室 liguoqiang@ouc.edu.cn 
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中文摘要:
      目的 研究一株采自西太平洋海山区深海沉积物中真菌Aspergillus versicolor(MCCC3A00937)的次级代谢产物。方法 利用薄层色谱法(TLC)、硅胶柱层析法、SephadexLH-20凝胶柱层析法、中压制备液相色谱法(MPLC)、高效液相色谱法(HPLC)等分离方法对该菌株发酵次级代谢产物进行了分离纯化；通过NMR、MS等现代波谱学方法,并结合相关文献进行数据分析,鉴定化合物结构。结果 从真菌Aspergillus versicolor(MCCC3A00937)的代谢产物中共分离得到9个化合物,分别是Averantin (1),Averantin-1"-meether (2),2H-6-O-Methylaverufin (3),6-O-Methylaverufin (4),6,8-di-O-methylnidurufin (5), versicolorin B (6),5-methoxysterigmatocystin (7),Brevianamide F (8), Psychrophilin E (9)。活性测试结果,化合物2对HL-60,BEL-704,HCT-116,A549,Hela和MCF-7细胞有一定的抑制作用,IC50分别为10.27,4.74,22.12,16.4,8.92,24.26 μM；化合物7对MCF-7细胞系有细胞毒性,IC50为12.25 μM。
English Summary:
      ObJective A secondary metabolite of fungus Aspergillus versicolor from the deep sea sediments of the Western Pacific seamounts was studied. Methods The secondary metabolites of the strain were separated and purified by thin layer chromatography (TLC), silica gel column chromatography, SephadexLH-20 gel column chromatography, medium pressure liquid chromatography (MPLC) and high performance liquid chromatography (HPLC). Results Nine compounds were isolated from the secondary metabolites of Aspergillus versicolor (MCCC3A00937), which were identified as Averantin (1), Averantin-1"-meether (2), 2H-6-O-Methylaverufin (3), 6-O-Methylaverufin (4), 6,8-di-O-methylnidurufin (5), versicolorin B (6), 5-methoxysterigmatocystin (7), Brevianamide F (8), Psychrophilin E (9). Among the compound 1~9, activity test results, compound 2 has inhibition on the tumor cells: the HL-60, BEL-704, HCT-116, A549, Hela and MCF-7, and the IC50 value were 10.27, 4.74, 22.12, 16.4, 8.92, 24.26 μM; compound 7 inhibited the growth of MCF-7 cells, and the IC50 value was 12.25 μM.
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