| 朱慧颖,周婉茹,张朝辉.海菖蒲黄酮FEA对LPS诱导RAW264.7细胞炎症反应的改善作用研究[J].中国海洋药物,2020,39(4):51-55. |
| 海菖蒲黄酮FEA对LPS诱导RAW264.7细胞炎症反应的改善作用研究 |
| Anti-inflammatory effect of flavonoids from Enhalus acoroides on RAW264.7 cells stimulated by LPS |
| 投稿时间：2020-02-28 修订日期：2020-03-27 |
| DOI： |
| 中文关键词: 海菖蒲黄酮FEA 抗炎症 炎症因子 NF-?B信号通路 |
| English Keywords:Enhalus acoroides flavonoids Anti-inflammatory Inflammatory factors NF-?B signal pathway |
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| 中文摘要: |
| 目的 研究海菖蒲黄酮FEA(Enhalus acoroides in flavonoids, FEA ) 对LPS刺激RAW264.7细胞引起的炎症反应的改善作用。方法 用浓度为1 μg/mL的LPS刺激RAW264.7细胞6个小时后,用含有不同浓度的海菖蒲黄酮FEA作用RAW264.7细胞20 h,采用ELISA方法测定RAW264.7细胞分泌的NO、TNF-α、IL-1β和IL-6的含量,采用western-blot方法检测NF-κB信号通路中IκBα、P-IκBα、p65、P-p65的蛋白表达含量。结果 海菖蒲黄酮FEA可以抑制由LPS刺激RAW264.7细胞引起的NO、TNF-α、IL-1β和IL-6细胞因子的分泌,减少磷酸化蛋白IκBα和p65的表达量。结论 海菖蒲黄酮FEA可以减少细胞炎症因子的产生和抑制NF-?B信号通路的活化来改善炎症作用。 |
| English Summary: |
| Abstract: Objective To study the anti-inflammatory activity of flavonoids from Enhalus acoroides induced by LPS on RAW264.7 cells. Methods After being stimulated with 1 μg/ml LPS for 6 h, RAW264.7 cells were further treated with different concentrations of flavonoids for additional 20 h. The amount of secreted NO, TNF-α, IL-1β and IL-6 were measured by ELISA method. The expression level of I?Bα, P-I?Bα, p65 and P-p65 were measured using western-blot method. Results The Enhalus acoroides flavonoids can inhibit the secretion of cytokines of NO, TNF-α, IL-1β and IL-6, and reduce the expression of phosphorylated proteins of I?Bα and p65. Conclusion The Enhalus acoroides flavonoids can exert anti-inflammatory effects by reducing the expression of cytokines and inhibiting activation of NF-?B signaling pathway. |
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