| 符萌,张雷,李雪静,等.鱼油和红花籽油对骨质疏松症小鼠脂肪酸合成的比较研究[J].中国海洋药物,2020,39(2):50-58. |
| 鱼油和红花籽油对骨质疏松症小鼠脂肪酸合成的比较研究 |
| The comparative study on the effect of fish oil and safflowerseed oil on fatty acid synthesis in ovariectomized mice |
| 投稿时间：2019-09-16 修订日期：2019-10-15 |
| DOI： |
| 中文关键词: 红花籽油 鱼油 脂肪酸合成 PPARγ NF-κB |
| English Keywords:safflowerseed oil fish oil fatty acid synthesis PPARγ NF-κB |
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| 中文摘要: |
| 摘要：骨质疏松患者通常伴发肥胖、高脂血症等脂代谢紊乱症状。鱼油（Fish Oil，FO）富含DHA、EPA等n-3 多不饱和脂肪酸（PUFA），红花籽油（Safflowerseed Oil，SO）富含亚油酸等n-6 PUFA，研究发现其对骨质疏松、动脉粥样硬化等多种疾病都有很好的调节作用，然而对骨质疏松症模型小鼠脂肪酸合成的作用尚未见报道。目的与方法 本实验采用双侧去卵巢手术建立骨质疏松症模型，研究了鱼油和红花籽油对模型小鼠脂肪酸合成的影响及作用机制。 结果 FO显著增加骨强度，降低模型小鼠体脂比、血清总胆固醇 （Total Cholesterol，TC）、甘油三酯（Triglyceride，TG）等脂代谢表观指标，降低n-6 PUFA代谢中间产物——前列腺素E2（Prostaglandin，PGE2）、前列腺素E受体4（Prostaglandin E receptor，EP4），以及下游炎症基因——核转录因子κB（Nuclear factor κB，NF-κB）、肿瘤坏死因子α（Tumor necrosis factor-α，TNF-α）的表达水平。由NF-κB介导的脂肪酸合成关键基因mRNA表达水平也显著下降：包括过氧化酶增殖因子活化受体γ（Peroxisome proliferator-activated receptor gamma，PPARγ）、固醇调节元件结合蛋白-1c（Sterol regulatory element binding protein-1c，SREBP1c）、脂肪酸合成酶（Fatty acid synthase，FAS）、乙酰辅酶A羧化酶（Acetyl-CoA carboxylase，ACC）。SO降低骨强度，显著提高模型小鼠体脂比、TC、PGE2含量以及脂肪酸合成调控基因SREBP1c的mRNA表达水平。 结论 提示通过介导PPARγ和NF-κB炎症通路，鱼油显著改善模型小鼠的脂质积累，而红花籽油则使模型小鼠的脂肪酸合成发生紊乱。 |
| English Summary: |
| Abstract: Patients with osteoporosis often have symptoms of lipid metabolism disorders, such as obesity and hyperlipidemia. Reports have shown that fish oil (FO, contained abundant n-3 polyunsaturated fatty acids (PUFAs), such as EPA/DHA) and safflower oil (SO, enriched n-6 PUFAs, especially linoleic) have a useful effect on osteoporosis, atherosclerosis and other diseases. However, the effect of FO/SO on fatty acid synthesis in osteoporosis mice have not been reported. Objective and Methods In this study, the mechanism of fish oil and safflower seed oil on fatty acid synthesis in osteoporosis mice induced by ovariectomized were investigated in vivo. Results FO significantly prevents bone loss, up-regulates the body fat percentage, total cholesterol (TC), triglyceride (TG), and reduces n-6 PUFAs metabolic intermediates-prostaglandin E2 (PGE2), Prostaglandin E receptor 4 (EP4), as well as the low expression levels of downstream inflammatory genes-nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α). The mRNA expression level of key genes involved in fatty acid synthesis mediated by NF-κB also decreased significantly, including peroxisome proliferator-activated receptor gamma (PPARγ), serol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), Acetyl-CoA carboxylase (ACC). SO significantly down-regulated bone mass, increased the body fat percentage, TC, PGE2 levels and the mRNA expression levels of SREBP-1c in fatty acid synthesis regulatory gene. Conclusion Fish oil significantly improved lipid accumulation while safflower seed oil caused fatty acid synthesis disorder in ovariectomized mice, which mediated by the PPARγ and NF-κB inflammatory pathway. |
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