| 徐艳,邹杰,谢一兴,等.广西北海方格星虫脂肪酸的提取及组成分析[J].中国海洋药物,2020,39(2):19-30. |
| 广西北海方格星虫脂肪酸的提取及组成分析 |
| Extraction and Fatty Acid Composition of Sipunculus nudus |
| 投稿时间：2019-09-12 修订日期：2019-10-11 |
| DOI： |
| 中文关键词: 方格星虫 脂质提取 脂肪酸组成 气相色谱-质谱联用法 |
| English Keywords:Sipunculus nudus lipid extraction fatty acid composition GC-M S |
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| 中文摘要: |
| 目的：比较分析方格星虫不同部位和极性层中的脂肪酸组成和含量。方法：采用气相色谱-质谱(GC-MS)联用法测定方格星虫(广西北海西村)不同部位和极性层的脂肪酸组成和含量。结果 方格星虫不同部位和极性层的脂肪酸含量和组成有差异,除了体腔液未鉴定出多不饱和脂肪酸和正丁醇层的单不饱和脂肪酸含量偏低以外,饱和脂肪酸都占总各自脂肪酸总量的一半左右,主要由 C14：0、C16：0 和 C18：0 所组成,单不饱和脂肪酸都占各自脂肪酸总量的 21.5-29.8 ％,其中 C18：1n9 和 C20：1n9 为主要成分,多不饱和脂肪酸占总脂肪酸的相对含量为 21.2-34.6 ％,其中 C20:4n6 的贡献最大,并且还含有一定量的 C20：5n3 (二十碳五烯酸 EPA)。结论 方格星虫体壁和石油醚层的脂肪酸提取率和鉴定出的脂肪酸比例最高,方格星虫脂肪酸不仅种类多样,还有具多种重要活性作用的不饱和脂肪酸。 |
| English Summary: |
| Objective: To study the fatty acids of different parts and polar layers from Sipunculus nudus and analyze its fatty acid composition. Methods: The GC-MS method was used to determine the fatty acids contents and compositions in different parts and polar layers of S. nudus. Results: The content and composition of fatty acids in different parts and polar layers of S. nudus were different. Apart from the content of monounsaturated fatty acids (MUFA)in n-butanol layer and the undetected poly unsaturated fatty acids(PUFA)in body fluid, the sums of saturated fatty acids (ΣSFA) were about half of the sum of total fatty acids (ΣTFA) , and the SFA was mainly composed of C16:0 and C18:0. The MUFA account for 21.5-29.8% of TFA, and the C18: 1n9 and C20：1n9 were the main component. The PUFA account for 21.2-34.6% of TFA, and was mainly composed of C18: 2n6 and C20:4n6, and it also contains a certain amount of EPA. Conclusion: The extraction rate of fatty acids from the body wall and petroleum ether layer of S. nudus was the highest, and the fatty acids of S. nudus were not only various, but also unsaturated fatty acids with many important activities. |
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