梅荣清,李灿,王斌,等.1株红树来源真菌Daldinia eschscholtzii HJ001 的活性次级代谢产物研究[J].中国海洋药物,2019,38(6):30-34.
1株红树来源真菌Daldinia eschscholtzii HJ001 的活性次级代谢产物研究
Studies on bioactive secondary metabolites from a mangrove-derived fungus Daldinia eschscholtzii HJ001
投稿时间：2019-08-01  修订日期：2019-08-30
DOI：
中文关键词:  药用红树  Daldinia eschscholtzii  次级代谢产物  抗氧化活性
English Keywords:medicinal mangrove plant  Daldinia eschscholtzii  secondary metabolites  antioxidant activity
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作者单位E-mail
梅荣清 海南师范大学 15707973425@163.com 
李灿 海南师范大学  
王斌 海南师范大学  
黄国雷 海南师范大学  
易继凌 海南师范大学  
陈光英 海南师范大学  
郑彩娟* 海南师范大学 caijuan2002@163.com 
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中文摘要:
      目的 研究1株红树尖瓣海莲来源内生真菌Daldinia eschscholtzii HJ001的次级代谢产物及其生物活性。方法 综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备HPLC等色谱分离技术对菌株的发酵产物进行分离纯化，通过NMR、MS、旋光等多种现代波谱技术以及与文献数据对照，确定化合物的结构，对化合物进行抗氧化活性和α-葡萄糖苷酶测试。结果 从红树来源内生真菌D. eschscholtzii HJ001中分离得到8个化合物（1~8），结构分别确定为sulochrin（1）、(4R)-O-methylsclerone（2）、(R)-(-)-mellein methyl ether（3）、helicascolide A（4）、kojic acid（5）、uracil（6）、cyclo（L-4-Hyp-L-pro）（7）、?ethyl β-D-xylopyranoside（8），除化合物4外，其余化合物均首次从Daldinia属中分离得到。?结论 化合物5显示强的抗氧化活性，IC50值为11.9 μmol·L?1，强于阳性对照Trolox（IC50 = 26.6 μmol·L?1）。
English Summary:
      Objective To investigate the bioactive secondary metabolites from the fermentation of mangrove Bruguiera sexangula var. rhynchopetala-derived fungus Daldinia eschscholtzii HJ001. Methods The secondary metabolites were isolated and purified by column chromatography over silica gel, Sephadex LH-20 and semi-preparative HPLC. Their structures were determined by NMR, MS, optical rotation and comparison with the data of literature. Their antioxidant and α-Glucosidase inhibitory activities were also tested. Results Eight compounds were isolated and identified as sulochrin (1), (4R)-O-methylsclerone (2), (R)-(-)-mellein methyl ether (3), helicascolide A (4), kojic acid (5), uracil (6), cyclo(L-4-Hyp-L-pro) (7) and ethyl-β-D-xylopyranoside (8). All compounds except 4 were isolated from the genus of Daldinia for the first time. Conclusion Compound 5 showed potent antioxidant activity (IC50 = 11.9 μmol·L?1) more active than the positive control Trolox (IC50 = 26.6 μmol·L?1).
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