| 陈润,林健,钟礼云,等.石房蛤毒素致突变性研究[J].中国海洋药物,2020,39(2):31-37. |
| 石房蛤毒素致突变性研究 |
| Study on mutagenicity of saxitoxin |
| 投稿时间：2019-05-24 修订日期：2019-11-11 |
| DOI： |
| 中文关键词: 石房蛤毒素 Ames试验 致突变性 CHL细胞 染色体 |
| English Keywords:saxitoxin Ames test mutagenicity CHL cell chromosome |
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| 中文摘要: |
| 摘 要：目的 通过Ames试验以及体外哺乳类细胞染色体畸变试验探索石房蛤毒素是否存在致突变性。 方法 通过Ames试验，选用组氨酸营养缺陷型鼠伤寒沙门氏菌TA97、TA98、TA100、TA102菌株，经过菌株鉴定及菌株毒性试验，在有无代谢活化系统存在的情况下分别对石房蛤毒素0.04、0.2、1.0 μg/皿三个剂量组进行试验，了解其是否具有致突变性。通过体外哺乳类细胞染色体畸变试验，采用MTT法检测石房蛤毒素对CHL细胞毒性，依据IC50，确定试验剂量后染毒，制片、吉姆萨染色，镜下观察染色体畸变数量及类型，计算畸变率，评价其致突变性。 结果 在有无S9活化系统条件下，在0.04、0.2、1.0 μg/皿测试浓度范围内，石房蛤毒素对测试菌株诱发产生的回变菌落数未达到溶剂对照组回变菌落数的2倍，且无剂量反应关系。石房蛤毒素对CHL细胞毒性测定结果显示，有S9时，其IC50值为1.20 μg/mL；无S9时，其IC50值为1.34 μg/mL。石房蛤毒素对CHL细胞染色体畸变作用的研究结果显示，各剂量组分别在加入S9混合液和未加S9混合液条件下染色体畸变数及畸变率与阳性对照组比较，存在显著性差异（P＜0.05）；与阴性对照组比较，均无显著性差异（P＞0.05）。结论 在本试验条件下，石房蛤毒素未见致突变性。 |
| English Summary: |
| Abstract: Objective To explore the mutagenicity of saxitoxin by Ames test and in vitro mammalian chromosome aberration test. Methods Through the Ames test, Select histidine auxotrophic Salmonella typhimurium strains TA97, TA98, TA100, TA102, After strain identification and strain toxicity test, Three dose groups of scorpion toxins 0.04, 0.2, 1.0 μg/dish were tested in the presence or absence of a metabolic activation system. Learn if it is mutagenic. Through in vitro mammalian cell chromosome aberration test, Toxicity of saxitoxin to CHL cells was detected by MTT assay. According to IC50, Identification of the test dose, exposure, tableting, Giemsa staining, The number and type of chromosome aberrations were observed under the microscope, the distortion rate was calculated, and the mutagenicity was evaluated. Results in the presence or absence of the S9 activation system, Within the test concentration range of 0.04, 0.2, 1.0 μg/dish, The number of reverting colonies induced by the testis strains was not twice as high as that of the solvent control group, and there was no dose-response relationship. The results of the determination of cytotoxicity of scorpion toxin on CHL showed that, When there is S9, its IC50 value is 1.20 μg/mL; When there is no S9, the IC50 value is 1.34 μg/mL. The results of the study on the chromosomal aberration of CHL cells by Shifang toxin showed that, The number of chromosome aberrations and the aberration rate of each dose group were compared with the positive control group under the condition of adding S9 mixture and no S9 mixture. There was a significant difference (P<0.05); Compared with the negative control group, There was no significant difference (P>0.05). Conclusion Under the conditions of this experiment, the saxitoxin was not mutagenic. |
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