文章摘要
海葵来源真菌Cochliobolus lunatus(TA26-46)对土霉素生物转化的研究
Biotransformation of the Anemone-Derived Fungus Cochliobolus lunatus on Oxytetracycline
投稿时间:2019-04-09  修订日期:2019-05-11
DOI:
中文关键词: 海葵来源真菌  Cochliobolus lunatus  土霉素  生物转化
英文关键词: anemone-derived fungus  Cochliobolus lunatus  oxytetracycline  biotransformation
基金项目:
作者单位E-mail
薛莹 中国海洋大学 海洋药物教育部重点实验室 18340851586@163.com 
石晓慧 中国海洋大学 海洋药物教育部重点实验室  
侯雪梅 中国海洋大学 海洋药物教育部重点实验室  
武京帅 中国海洋大学 海洋药物教育部重点实验室  
王长云 中国海洋大学 海洋药物教育部重点实验室 changyun@ouc.edu.cn 
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中文摘要:
      目的 研究海葵来源真菌Cochliobolus lunatus (TA26-46)对土霉素的生物转化作用。方法 通过在察氏固体培养基平板中添加土霉素,利用海洋真菌体内特殊的转化酶系统对土霉素进行生物转化;利用HPLC-DAD进行转化产物的追踪,并运用硅胶柱层析和半制备型HPLC等分离纯化转化产物,利用核磁、质谱等现代波谱分析方法对转化产物进行结构鉴定,并测试转化产物的抗菌活性。 结果 海洋真菌C. lunatus (TA26-46)对土霉素(1)产生了生物转化作用,从发酵物中分离鉴定得到2个土霉素的降解产物hemi-cyclines A 和B (2和3)。抗菌活性测试结果表明,降解产物未显示抗菌活性。结论 通过生物学方法,利用海洋真菌C. lunatus (TA26-46)成功对四环类抗生素土霉素进行了生物转化,获得了无抗菌活性的降解产物,为解决环境中的抗生素污染问题提供了借鉴。
英文摘要:
      Objective To study the biotransformation of oxytetracycline by anemone -derived fungus Cochliobolus lunatus (TA26-46). Methods Oxytetracycline was biotransformed by using a special invertase system in marine fungi by adding oxytetracycline to the solid medium plates. The conversed products were traced by HPLC-DAD and separated and purified by silica gel column chromatography and semi-preparative HPLC. The transformation products were identified by comprehensive spectroscopy including nuclear magnetic resonance, mass spectrometry. The antibacterial activities of the transformed products were tested. Results The oxytetracycline (1) was biotransformed by marine fungus C. lunatus (TA26-46) successfully. Two oxytetracycline degradation products, hemi-cyclines A and B (2 and 3), were isolated and identified from the fermentation culture. The antibacterial activity assay showed that the degradation products did not exhibit antibacterial activity. Conclusion Through the biological method, the tetracyclic antibiotic, oxytetracycline, was successfully biotransformd by marine fungus C. lunatus (TA26-46), leading to the discovery of degradation products without antibacterial activity. Our study provided a reference for solving the problem of antibiotic pollution in the environment.
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