董尧,王晓梅,唐旭利,等.1株软珊瑚共附生真菌Penicillium sclerotiorum中azaphilones类化合物及其生物活性研究[J].中国海洋药物,2019,38(6):15-20.
1株软珊瑚共附生真菌Penicillium sclerotiorum中azaphilones类化合物及其生物活性研究
Studies on azaphilones and their bioactivities of the fungus Penicillium sclerotiorum isolated from soft coral species
投稿时间：2019-03-01  修订日期：2019-04-29
DOI：
中文关键词:  Penicillium sclerotiorum  次级代谢产物  azaphilone  生物活性
English Keywords:Penicillium sclerotiorum  secondary metabolite  azaphilone  biological activity
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作者单位E-mail
董尧* 中国海洋大学海洋药物教育部重点实验室 2456510718@qq.com 
王晓梅 中国海洋大学海洋药物教育部重点实验室  
唐旭利 中国海洋大学化学化工学院  
李平林 中国海洋大学海洋药物教育部重点实验室  
李国强 中国海洋大学海洋药物教育部重点实验室  
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中文摘要:
      目的对1株西沙群岛永兴岛海域来源短指软珊瑚sinularia sp.共附生真菌Penicillium sclerotiorum中的azaphilones类化合物及其生物活性进行研究。方法利用硅胶柱层析、薄层色谱、Sephadex LH-20凝胶柱层析、MPLC、HPLC等分离手段对菌株发酵提取物进行分离和纯化；运用NMR、MS等现代波谱学方法,并结合相关文献数据比对,鉴定化合物的结构；通过细胞毒活性模型评价化合物抗肿瘤活性。结果从真菌Penicillium sclerotiorum的代谢产物中获得了9个azaphilones类合物,分别(+)-Sclerotiorin(1), Isochromophilone Ia(2), Isochromophilone Ib(3), Isochromophilone III(4), epi-isochromophilone III (5), Isochromophilone IV(6), dechloroisochromophilone IV(7), Isochromophilone VIII(8), TL-1-monoAc(9),其中化合物8在30μmol.L-1浓度下对HL-60细胞的抑制率达到97.87%,IC50值为11.81μmol.L-1。
English Summary:
      Objective To study the azaphilones compounds of the epiphytic fungus Penicillium sclerotiorum from the Xisha Islands soft coral sinularia sp., Methods The fermentation extracts of the strains were separated and purified by silica gel column chromatography, thin layer chromatography, Sephadex LH-20 gel column chromatography, MPLC and HPLC. The spectroscopy methods such as NMR and MS were used. The literature data was aligned to identify the structure of the compound; The antitumor activities of the compounds were evaluated by a cytotoxicity experiments. Results Nine azaphilones were isolated from the fungus Penicillium sclerotiorum, namely (+)-Sclerotiorin (1), Isochromophilone Ia (2), Isochromophilone Ib (3), Isochromophilone III (4), epi-isochromophilone III(5), Isochromophilone IV(6), dechloroisochromophilone IV(7), Isochromophilone VIII (8), TL-1-monoAc (9). Compound 8 inhibited HL-60 cells at 30 μmol.L-1by 97.87%. The IC50value is 11.81μmol.L-1.
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