文章摘要
海藻多糖UH3的结构特征、抗凝和溶栓活性研究
Structural characterization, anticoagulant and thrombolytic activities of the seaweed polysaccharide UH3
投稿时间:2019-01-08  修订日期:2019-03-05
DOI:
中文关键词: 海藻多糖  结构特征  抗凝血活性  溶栓活性
英文关键词: seaweed polysaccharide  structural characterization  anticoagulant activity  thrombolytic activity
基金项目:国家自然科学基金项目(41476108);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ08,2015ASKJ02);国家自然科学基金委员会-山东省人民政府联合资助海洋科学研究中心项目(U1606403);
作者单位E-mail
曹素健 中国海洋大学医药学院 海洋药物教育部重点实验室 846189507@qq.com 
杨亚靖 中国海洋大学医药学院 海洋药物教育部重点实验室  
秦岭 中国海洋大学医药学院 海洋药物教育部重点实验室  
何美佳 中国海洋大学医药学院 海洋药物教育部重点实验室  
付子桃 中国海洋大学医药学院 海洋药物教育部重点实验室  
毛文君 中国海洋大学医药学院 海洋药物教育部重点实验室 wenjunm@ouc.edu.cn 
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中文摘要:
      目的 对海藻多糖UH3的结构特征、抗凝血和溶栓活性进行研究。方法 通过热水提取法,从海藻中提取硫酸多糖,采用强阴离子交换色谱和凝胶渗透色谱对多糖进行分离纯化;采用高效液相色谱、高效凝胶渗透色谱(HPGPC)、红外光谱及甲基化方法对多糖的结构进行表征;通过活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、凝血酶原时间(PT)及血凝块溶解法研究多糖体外抗凝血和溶栓活性。结果 多糖UH3在HPGPC色谱图上呈单一对称峰,分子量为50.3 kDa,硫酸根和糖醛酸含量分别为12.27和12.85%;UH3主要由鼠李糖组成,含有少量葡萄糖醛酸和木糖;UH3糖链中鼠李糖主要以→4)-Rhap-(1→、 →3,4)-Rhap-(1→及少量的T-Rhap形式存在,木糖以→4)-Xylp-(1→和T-Xylp存在形式,糖醛酸以→4)-GlcAp-(1→和T-GlcAp形式存在;硫酸根主要位于→4)-Rhap-(1→的C-3位。多糖UH3对APTT和TT有显著延长作用,并能明显提高溶栓率。结论 海藻多糖UH3是一种结构新颖的具有抗凝和溶栓活性的硫酸多糖。
英文摘要:
      Objective To investigate the structural characterization, anticoagulant and thrombolytic activity of the seaweed polysaccharide UH3. Methods The crude polysaccharide was extracted from green alga by hot water, and further purified by strong anion exchange chromatography and gel permeation chromatography. The structure of the polysaccharide was characterized by high performance liquid chromatography, high performance gel permeation chromatography (HPGPC), Fourier infrared spectroscopy (FT-IR) and methylation analysis. The anticoagulant and thrombolytic activities of the polysaccharide were determined by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and clot lytic assay in vitro. Results The polysaccharide of UH3 appeared as a single peak in the HPGPC chromatogram, and its average molecular weight was about 50.3 kDa. The contents of sulfate esters and uronic acid were 12.27 and 12.85%, respectively. The polysaccharide UH3 was mainly composed of rhamnose with small amounts of glucuronic acid and xylose. The rhamnose in polysaccharide UH3 was existed in the forms of →4)-Rhap-(1→, →3,4)-Rhap-(1→ and small amount of T-Rhap, the xylose was existed as →4)-Xylp-(1→ and T-Xylp, and the glucuronic acid was existed as →4)-GlcAp-(1→ and T-GlcAp. The sulfate ester was substituted at the C-3 of →4)-Rhap-(1→ residues. Polysaccharide UH3 could significantly prolonged APTT and TT, and significantly increased clot lytic rate in vitro. Conclusion The seaweed polysaccharide UH3 was a novel sulfated polysaccharide with anticoagulant and thrombolytic activities.
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