| 买小圆,唐薇,戚欣,等.柳珊瑚来源的愈创木薁倍半萜生物碱化合物Muriceidine A体外抗肿瘤活性的研究[J].中国海洋药物,2019,38(3):26-32. |
| 柳珊瑚来源的愈创木薁倍半萜生物碱化合物Muriceidine A体外抗肿瘤活性的研究 |
| Anti-tumor activity in vitro of a novel guaiazulene sesquiterpene alkaloid Muriceidine A from the gorgonian Muriceides collaris |
| 投稿时间：2018-12-07 修订日期：2019-01-09 |
| DOI： |
| 中文关键词: Muriceidine A HeLa细胞 增殖抑制 周期阻滞 凋亡诱导 |
| English Keywords:Muriceidine A HeLa cells proliferation inhibition cycle arrest apoptosis |
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| 中文摘要: |
| 目的 对从高领类尖柳珊瑚Muriceides collaris中分离得到的1种愈创木薁倍半萜生物碱Muriceidine A进行体外抗肿瘤活性研究。方法 MTT法测定Muriceidine A对多种肿瘤细胞株的增殖抑制作用。流式细胞仪检测Muriceidine A对HeLa细胞周期及凋亡的影响。Western Blot检测Muriceidine A对HeLa细胞中周期相关蛋白、凋亡相关蛋白、E6/E7蛋白及其下游信号分子的影响。结果 Muriceidine A体外对多种肿瘤细胞均有增殖抑制作用，其中对HeLa 细胞的抑制作用最强，IC50为17.40 μmol/L。流式细胞仪分析及Western Blot检测显示，Muriceidine A 能够以剂量依赖性的方式使HeLa细胞阻滞在G2/M期，可通过激活Caspase依赖的线粒体凋亡途径诱导HeLa细胞凋亡。同时，Western Blot检测显示Muriceidine A能够通过降低HeLa细胞中E6/E7的水平，抑制ERK和STAT3的磷酸化。结论 Muriceidine A体外对人宫颈腺癌细胞HeLa具有较强的细胞毒作用。本研究为该小分子化合物今后的合成与改造提供思路与方向。 |
| English Summary: |
| Objective To investigated the anti-tumor activity in vitro of a novel compound Muriceidine A, which is a novel guaiazulene sesquiterpene alkaloid isolated from the gorgonian Muriceides collaris. Methods MTT assay was used to determine the proliferation inhibition effect of Muriceidine A on various tumor cells. Flow cytometry was used to detect the effect of Muriceidine A on HeLa cell cycle and apoptosis. The effects of Muriceidine A on cycle-associated proteins, apoptosis-related proteins, E6/E7 proteins and downstream signaling molecules in HeLa cells were assessed by Western Blot. Results Muriceidine A inhibited the proliferation of various tumor cells in vitro, and the inhibitory effect on human cervical cancer cell HeLa was the strongest, with an IC50 of 17.40 μmol/L. Flow cytometry analysis and Western Blot assay showed that Muriceidine A could arrest HeLa cells in G2/M phase in a dose-dependent manner, and induce HeLa cell apoptosis by activating Caspase-dependent mitochondrial apoptosis pathway. Meanwhile, Western Blot assay showed that Muriceidine A inhibited the phosphorylation of ERK and STAT3 by reducing the level of E6/E7 in HeLa cells. Conclusion Muriceidine A had a strong cytotoxic effect on human cervical cancer cell line HeLa in vitro. The anti-tumor activity in vitro of Muriceidine A provided new ideas and directions for the future synthesis and transformation of it. |
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