文章摘要
1株海洋来源真菌Penicillium chrysogenum生物活性成分研究
Studies on bioactive components of a marine-derived fungus Penicillium chrysogenum
投稿时间:2018-11-26  修订日期:2018-12-20
DOI:
中文关键词: 共附生真菌  P.chrysogenum  次级代谢产物
英文关键词: epiphytic fungi  P.chrysogenum  secondary metabolites
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
韩箫 中国海洋大学 海洋药物教育部重点实验室 1156642014@qq.com 
唐旭利 中国海洋大学 海洋药物教育部重点实验室  
乔丹 中国海洋大学 海洋药物教育部重点实验室  
李平林 中国海洋大学  
李国强 中国海洋大学 海洋药物教育部重点实验室 liguoqiang@ouc.edu.cn 
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中文摘要:
      摘 要:目的 研究一株中国西沙群岛永兴海域简易板海绵Plakortis simplex共附生真菌Penicillium chrysogenum(14XS09-2)的次级代谢产物。方法 利用薄层色谱、硅胶色谱、Sephadex LH-20凝胶柱色谱、ODS中压色谱、半制备HPLC等方法对次级代谢产物进行了分离纯化;通过NMR、MS等方法进行进一步的数据分析,与相关文献结合,鉴定化合物结构。结果 从真菌Penicillium chrysogenum(14XS09-2)中分离得到10个化合物,分别为isoroquefortine C(1),meleagrin(2),2",3"-dihydrosorbicillin(3),sohirnone A(4),sorbicillin(5),rezishanones D(6),N-(2-methoxyphenyl)(7),2-苯并唑啉酮(8),cyclo-(L-Try-L-Pro)(9),Nb-acetyltryptamine(10)。结论 化合物3和4在40 μmol·L?1浓度水平,对PTP1B的活性抑制率分别是10.58%、8.47%。
英文摘要:
      Abstract: Objective To investigate the secondary metabolites of the Penicillium chrysogenum (14XS09-2) isolated from a simple plate sponge Plakortis simplex collected from the Yongxing sea area of Xisha Islands, China. Methods The secondary metabolites were purified by TLC and column chromatography over silica gel, Sephadex LH-20, ODS and HPLC, and their structures were determined by spectroscopic analysis including NMR and MS,and by comparison with the data of literature. Results Ten compounds were isolated from the secondary metabolites of Penicillium chrysogenum (14XS09-2), which were identified as isoroquefortine C(1), meleagrin(2), 2", 3"-dihydrosorbicillin (3), sohirnone A(4 ), sorbicillin (5), rezishanones D (6), N-(2-methoxyphenyl)(7), 2-benzoxazolinone (8), cyclo-(L-Try-L-Pro) (9), Nb-acetyltryptamine (10). Conclusion Compounds 3 and 4 exhibited moderate cytotoxicity against PTP1B with the proliferation inhibition rate of 10.58% and 8.47%, respectively, at the concentration level of 40 μmol·L?1.
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