| Abstract: Objective The studied polypeptides activities in antibacterial and antithrombotic activities were extracted from sargassum. Method The polypeptide components were isolated and purified by sephdexg-50 gel chromatography and HPLC, and the samples were monitored at 280 nm. Molish experiment detected the sugar content of polypeptide. Effects of different doses of sargassum polypeptide (0, 10, 20, 40, 60, 80 g/mL) on growth of Escherichia coli (D1314) and Staphylococcus aureus (s.agr+, RN4220) were detected by using the method of turbidity. Rats were randomly divided into normal saline group, polypeptides (100, 200, 400 g/mL) and heparin (1600U/mL) groups, and given corresponding drugs, respectively, to electrically stimulate the carotid thrombosis , and observe the situation of carotid thrombosis .
Results Two collection peaks can be obtained from the supernatant of sargassum, the main polypeptides were concentrated in the B collecting peak.The analysis of B collection peak consists of 6 components by HPLC. Molish experiments showed that the polypeptide components did not contain polysaccharides. The polypeptide can inhibit the growth of Escherichia coli, and is not sensitive to Staphylococcus aureus. By electrical stimulation of carotid thrombosis in rats, it was found that the polypeptide could inhibit thrombosis.
Conclusion We have successfully extracted the active polypeptide from Sargassum , which can inhibit the growth and thrombosis of Escherichia coli.