文章摘要
中国南海海绵Plakortis sp.次生代谢产物研究
Study on the secondary metabolites of sponge Plakortis sp.
投稿时间:2018-11-01  修订日期:2018-12-22
DOI:
中文关键词: 扁板海绵  Plakortis sp.  化学成分  结构鉴定  聚酮化合物
英文关键词: sponge Plakortis sp.  chemical constituents  structure identification  polyketides
基金项目:国家自然科学(21302005, 21672010)资助
作者单位E-mail
季宇彬 哈尔滨商业大学 生命科学与环境科学研究中心 381706145@qq.com 
陈琪通 哈尔滨商业大学 生命科学与环境科学研究中心  
程伟 北京大学 天然药物及仿生药物国家重点实验室  
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中文摘要:
      摘要:目的 研究海绵Plakortis sp.的次生代谢产物及其生物活性。方法 利用硅胶、HPLC等色谱学方法对海洋动物Plakortis sp.海绵进行分离纯化。通过NMR、MS、旋光等光谱学方法,并结合文献数据,确定化合物的结构。并对分离得到的化合物进行细胞毒,抗菌和抗HBV活性测试。 结果 共分离得到4个聚酮类化合物,结构确定为化合物1 6-desmethyl-6-ethyl-spongosoritin A、化合物2 6-desmethyl-6-ethyl-9,10-dihydrospongosoritin A、化合物3 methyl(2Z, 6R, 8S)-4,6-diethyl-3,6-3poxy-8-methyldodeca-2,4-dienoate、化合物4 spongosoritin A。 结论 化合物1~4为该海绵特征聚酮类代谢产物,细胞毒,抗菌和抗HBV活性结果显示,化合物1~4对金黄色葡萄球菌和大肠杆菌无生长抑制作用,对HCT-8、MCF-7、SMMC-7和HepG-2这四种癌细胞基本无生长抑制作用,化合物1~3基本无抗HBV活性,但化合物4表现出强烈的抗HBV活性。
英文摘要:
      Abstract: Objective To investigate the chemical constituents and bioactivity of the secondary metabolites from aa sponge of Plakortis sp. collected from the South China Sea. Methods The compounds were isolated and purified by silica gel column chromatography and HPLC. Their structures were identified by NMR,MS,optical rotation and comparison with the data of literature. The antibacterial activities, anti-HBV activities and cytotoxicities of all the compounds were tested. Results Four compounds were isolated from the EtOAc extract and identified as compound 1 6-desmethyl-6-ethyl-spongosoritin A, compound 2 6-desmethyl-6-ethyl-9,10-dihydrospongosoritin A, compound 3 methyl(2Z, 6R, 8S)-4,6-diethyl-3,6-3poxy-8-methyldodeca-2,4-dienoate, compound 4 spongosoritin A. Conclusion Compounds 1~4 were characteristic polyketides from this sponge and all the compounds showed no antibacterial activity and weak cytotoxicity to HCT-8, MCF-7, SMMC-7 and HepG-2. Furthermore, compounds 1~3 showed no anti-HBV activitiy, but compound 4 showed potent anti-HBV activity.
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