| Objective To evaluate the hemostatic property and wound healing effects of new electrostatic spinning wound dressing, and lay a foundation for its further development and application. Methods The new electrostatic spinning wound dressing (collagen-chitosan electrostatic spinning membrane alginate dressing，CCEAD) was prepared by spinning the mixture of collagen and chitosan onto alginate non-woven fabric substrate by electrospinning technology. The hemostatic properties of CCEAD were evaluated by Blood Clotting Index (BCI) test, rabbit ear artery trauma hemostasis test and rabbit back trauma hemostasis test. Rat back wound model was used to evaluate the wound healing effects. The wound healing rate was calculated by taking pictures on the 3rd, 5th, 7th and 12th day after operation. HE staining was used to observe histopathological changes. Immunohistochemical staining was used to observe CD31, FGF-2 and TGF-β expression. Results The BCI of CCEAD group was 36.91±6.62, which was significantly less than gelatin sponge group (69.82±1.83) and gauze group (79.93±6.30) (P＜0.01). In the hemostasis test of rabbit ear artery trauma, the hemostatic time of CCEAD group (107.67±17.62) s was significantly shorter than that of gauze group (163.67±15.50) s (P＜0.05). In the hemostasis test of rabbit back trauma, the absorbance value of Hb in CCEAD group (0.120±0.052) was significantly lower than that of gauze group (0.294±0.021) (P＜0.01). In the wound healing test, on the 7th day after operation, the healing rate of CCEAD group was 65.70%±4.94%, which was higher than that of chitosan dressing group (48.78%±8.33%,P＜0.05) and significantly higher than gauze group (37.76%±6.35%,P＜0.01). HE staining showed that CCEAD could promote fibroblast growth, angiogenesis and granulation tissue formation in the early stage of wound healing. Immunohistochemical results showed that CCEAD could promote the expression of CD31, FGF-2 and TGF-beta in the early stage of wound healing, which was beneficial to wound healing. Conclusion The new electrostatic spinning wound dressing (collagen-chitosan electrostatic spinning membrane alginate dressing) has excellent hemostatic properties and obvious effect of promoting wound healing. It is expected to be developed into a new wound dressing.