| 王昭晶,罗巅辉,聂开颖.网格状与线状鼠尾藻多糖抗炎作用研究[J].中国海洋药物,2019,38(2):17-23. |
| 网格状与线状鼠尾藻多糖抗炎作用研究 |
| Anti-inflammation Effects of Linear and Mesh-shaped Polysaccharides from Sargassum thunbergii |
| 投稿时间：2018-10-23 修订日期：2019-01-14 |
| DOI： |
| 中文关键词: 鼠尾藻 微观结构 抗炎活性 透射电子显微镜 |
| English Keywords:Sargassum thunbergii microstructure anti-inflammation effects TEM |
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| 中文摘要: |
| 目的 对鼠尾藻多糖进行分离纯化，并对其理化特性、微观结构和对炎症因子表达的影响进行研究。方法 采用热水提取法及DEAE-Sepharose CL-6B和Sephacryl S-300 HR柱层析分离纯化鼠尾藻多糖，利用高效空间排阻色谱法（HPSEC）、气相色谱法（GC）、红外光谱法（FTIR）和透射电子显微镜（TEM）研究理化特性及微观结构，实时荧光定量PCR法研究炎症相关因子的mRMA表达。结果 得到两种鼠尾藻均一多糖STP-I和STP-III，相对分子质量分别是29 和350 kDa，糖含量均超过96%。透射电子显微镜观察到STP-I是网格状结构，STP-III是线状且螺旋缠绕结构。活性研究表明，STP-III能更好地抑制脂多糖（LPS）诱导下小鼠巨噬细胞RAW264.7系炎症因子TNF-α、IL-6和COX-2的mRNA表达量，在150 μg?mL1质量浓度下，STP-III对炎症因子TNF-α、IL-6和COX-2的mRNA表达抑制率均超过95%。结论 鼠尾藻多糖具有抗炎症因子表达的作用，可以作为潜在的抗炎药物开发。线状缠绕的鼠尾藻多糖STP-III较网格状的STP-I能更好地抑制炎症相关因子的表达。 |
| English Summary: |
| OBJECTIVE To isolate polysaccharides from Sargassum thunbergii and study their physicochemical properties, microstructures and anti-inflammation effects. METHODES Two polysaccharides (STP-I and STP-III) were purified using water extraction method, DEAE-Sepharose CL-6B and Sephacryl S-300 HR. The physicochemical properties and microstructures were studied using high-performance size-exclusion chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analysis. Anti-inflammation activities of the polysaccharides were identified using real time quantitative PCR. RESULTS STP-I and STP-III were all homogeneous, molecular weights (Mw) were 29 and 350 kDa, and the percentage contents of total carbohydrates were more than 96%. TEM revealed further that STP-I was mesh-shaped, and STP-III was observed as linear microstructures with entangled chains. Due to the results of anti-inflammation activities of the polysaccharides, STP-III showed the higher inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells than others, and the inhibitory effects were more than 95% at 150 μg . ml-1 concentrations. CONCLUSION The polysaccharides from S. thunbergii have obvious anti-inflammation effects and may serve as potential anti-inflammatory agents, and linear STP-III shows more noticeable anti-inflammation effects than the huge mesh-shaped STP-I. |
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