文章摘要
网格状与线状鼠尾藻多糖抗炎作用研究
Anti-inflammation Effects of Linear and Mesh-shaped Polysaccharides from Sargassum thunbergii
投稿时间:2018-10-23  修订日期:2019-01-14
DOI:
中文关键词: 鼠尾藻  微观结构  抗炎活性  透射电子显微镜
英文关键词: Sargassum thunbergii  microstructure  anti-inflammation effects  TEM
基金项目:
作者单位E-mail
王昭晶 华侨大学 zhaojingwang1203@qq.com 
罗巅辉 华侨大学 dianhuiluo@163.com 
聂开颖 华侨大学  
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中文摘要:
      目的 对鼠尾藻多糖进行分离纯化,并对其理化特性、微观结构和对炎症因子表达的影响进行研究。方法 采用热水提取法及DEAE-Sepharose CL-6B和Sephacryl S-300 HR柱层析分离纯化鼠尾藻多糖,利用高效空间排阻色谱法(HPSEC)、气相色谱法(GC)、红外光谱法(FTIR)和透射电子显微镜(TEM)研究理化特性及微观结构,实时荧光定量PCR法研究炎症相关因子的mRMA表达。结果 得到两种鼠尾藻均一多糖STP-I和STP-III,相对分子质量分别是29 和350 kDa,糖含量均超过96%。透射电子显微镜观察到STP-I是网格状结构,STP-III是线状且螺旋缠绕结构。活性研究表明,STP-III能更好地抑制脂多糖(LPS)诱导下小鼠巨噬细胞RAW264.7系炎症因子TNF-α、IL-6和COX-2的mRNA表达量,在150 μg?mL1质量浓度下,STP-III对炎症因子TNF-α、IL-6和COX-2的mRNA表达抑制率均超过95%。结论 鼠尾藻多糖具有抗炎症因子表达的作用,可以作为潜在的抗炎药物开发。线状缠绕的鼠尾藻多糖STP-III较网格状的STP-I能更好地抑制炎症相关因子的表达。
英文摘要:
      OBJECTIVE To isolate polysaccharides from Sargassum thunbergii and study their physicochemical properties, microstructures and anti-inflammation effects. METHODES Two polysaccharides (STP-I and STP-III) were purified using water extraction method, DEAE-Sepharose CL-6B and Sephacryl S-300 HR. The physicochemical properties and microstructures were studied using high-performance size-exclusion chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analysis. Anti-inflammation activities of the polysaccharides were identified using real time quantitative PCR. RESULTS STP-I and STP-III were all homogeneous, molecular weights (Mw) were 29 and 350 kDa, and the percentage contents of total carbohydrates were more than 96%. TEM revealed further that STP-I was mesh-shaped, and STP-III was observed as linear microstructures with entangled chains. Due to the results of anti-inflammation activities of the polysaccharides, STP-III showed the higher inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells than others, and the inhibitory effects were more than 95% at 150 μg . ml-1 concentrations. CONCLUSION The polysaccharides from S. thunbergii have obvious anti-inflammation effects and may serve as potential anti-inflammatory agents, and linear STP-III shows more noticeable anti-inflammation effects than the huge mesh-shaped STP-I.
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