| 郑玉冰,汤华,宋克义,等.东沙珊瑚共附生真菌ZH-IV-2的活性成分研究[J].中国海洋药物,2018,37(5):21-29. |
| 东沙珊瑚共附生真菌ZH-IV-2的活性成分研究 |
| Research of the bioactive compounds from coral-associated fungus fungus ZH-IV-2 |
| 投稿时间：2018-07-24 修订日期：2018-08-20 |
| DOI： |
| 中文关键词: 珊瑚共附生真菌 真菌ZH-IV-2 缩酚酸环醚 环肽 |
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| 中文摘要: |
| 摘要:目的 研究软珊瑚Sarcophyton sp.共附生真菌ZH-IV-2中的活性成分。方法 采用正相硅胶柱色谱、反相ODS柱色谱、凝胶Sephadex LH-20柱色谱以及高效液相色谱（HPLC）等分离手段对真菌ZH-IV-2的乙酸乙酯提取物分离纯化；并通过理化性质、波谱学数据等信息结构并文献报道数据鉴定化合物结构；采用羧基荧光素琥珀酰亚胺酯(CFSE)流式细胞法和酒石酸抗性酸磷酸酶（TRAP）染色法体外评价化合物对原代淋巴细胞的免疫调节活性及对髓系巨噬细胞的破骨细胞分化活性。结果 分离得到7个已知化合物，结构分别鉴定为：aspergillusidone B(1)及C(2)，emeguisin A(3)，unguinol(4)，diorcinol(5)，6-butyl-3-methyl-(3R,3aR,6S,6aS)-perhydrofuro[3,4-b]furan-2,4-dione(6)，unguisin A(7)。在体外活性测试中，化合物3在0.5和1 μmol/L时对淋巴细胞增殖有明显的促进作用，化合物6在0.5和1 μmol/L时对破骨细胞生成有明显的促进作用，结果均具有显著性差异。结论报道化合物4的13C-NMR核磁数据，并对化合物7核磁数据归属进行校正。首次对化合物3的免疫调节活性及化合物6的破骨前体细胞分化活性进行报道，具有进一步研究价值。 |
| English Summary: |
| Abstract: Objective To investigate the bioactive chemical constituents of fungus ZH-IV-2 from the soft coral Sarcophyton sp. Methods The EtOAc extract of the fungus ZH-IV-2 was purified by repeated column chromatography on silica gel, Sephadex LH-20, and RP-HPLC. The structures of the compounds were determined based on spectroscopic analysis and comparison with reported data. The compounds were evaluated in vitro for their immunomodulatory activity on primary lymphocytes and osteoclastogenesis inhibitory activity on myeloid macrophages using 5-Carboxy-fluorescein diacetate N-succinimidyl ester (CFSE) and tartrate resistant acid phosphatase (TRAP) staining methods, respectively. Results Seven known compounds were isolated and identified as aspergilluidones B (1) and C (2) , emeguisin A (3) , unguinol (4) , diorcinol (5) and 6-butyl-3-methyl-(3R,3aR,6S,6aS)-perhydrofuro[3,4-b]furan-2,4-dione (6), and unguisin A (7) . Compound 3 can significantly promote T cell proliferation at 0.5 and 1 μmol/L, while compound 6 can significantly induce osteoclastogenesis at 0.5 and 1 μmol/L. Conclusion Compound 4 was reported for its 13C-NMR data. The NMR assignments for compound 7 were corrected. This is the first report of immunomodulatory activity of 3 and the osteoclastogenesis activity of 6. The research may promote further investigations on the metabolites of these kinds. |
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